Reactive Carbon Nano-Onion Modified Glassy Carbon Surfaces as DNA Sensors for Human Papillomavirus Oncogene Detection with Enhanced Sensitivity,Analytical Chemistry

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Reactive Carbon Nano-Onion Modified Glassy Carbon Surfaces as DNA Sensors for Human Papillomavirus Oncogene Detection with Enhanced Sensitivity
Analytical Chemistry ( IF 6.7 ) Pub Date : 2015-06-22 00:00:00 , DOI: 10.1021/acs.analchem.5b00924
Joanne P. Bartolome ₁ , Luis Echegoyen ₂ , Alex Fragoso ₁

Affiliation

Glassy carbon electrodes were modified with small carbon nano-onions (CNOs) and activated by electrografting of diazonium salts bearing terminal carboxylic acid and maleimide groups. The CNO-modified surfaces were characterized by ESEM and AFM microscopy as well as by electrochemical techniques. The modified electrodes were used for the amperometric detection of a model DNA target sequence associated with the human papillomavirus by immobilizing short recognition sequences by amidation or thiol-maleimide reactions. The analytical parameters of the developed biosensors were compared with glassy carbon electrodes without CNOs. In both cases, the incorporation of CNOs resulted in an enhancement in sensitivity and a decrease in detection limits ascribed to a combination of large surface areas and enhanced electron transfer properties of the CNO-modified electrodes. These results offer promise for the construction of other CNO-based biomolecule detection platforms with enhanced sensitivities.

中文翻译：

活性碳纳米洋葱修饰的玻碳表面作为人类乳头瘤病毒致癌基因检测的DNA传感器，具有增强的灵敏度

玻碳电极用小碳纳米离子（CNO）修饰，并通过电接枝带有末端羧酸和马来酰亚胺基团的重氮盐活化。通过ESEM和AFM显微镜以及电化学技术对CNO改性的表面进行了表征。修饰的电极通过酰胺化或硫醇马来酰亚胺反应固定短识别序列，用于安培检测与人乳头瘤病毒相关的模型DNA靶序列。将开发的生物传感器的分析参数与不含CNO的玻璃碳电极进行了比较。在这两种情况下 CNO的掺入导致灵敏度的提高和检测限的降低，这归因于CNO修饰电极的大表面积和增强的电子转移性能的组合。这些结果为构建具有更高灵敏度的其他基于CNO的生物分子检测平台提供了希望。

更新日期：2015-06-22

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