Expanded polystyrene (EPS) has caused significant pollution in marine environments, with potential EPS-degrading bacteria identified on long-term floating EPS biofilms. However, studies on bacterial interactions and consortium reconstruction based on in-situ bacterial diversity remain limited. Marine EPS wastes of different sizes were collected from subtropical coast of Xiamen island, and subjected to bacterial diversity analyses. Co-occurrence network and bacterial characterization revealed that Rhodobacterales and Rhizobiales play important roles in polystyrene (PS) degradation. Bacterial isolation characterization confirmed that Fulvimarina pelagi, Pseudosulfitobacter pseudonitzschiae, Devosia nitrariae, Cytobacillus kochii, and Cytobacillus oceanisediminis as novel PS-degraders. Based on their abundance in situ and PS degradation activity, a consortium was constructed, constituted of F. pelagi, P. halotolerans. and O. granulosus, showed a high degradation capability with PS weight loss by 18.9% in 45 days. These results contribute to marine plastic pollution remediation and resources recycling.

中文翻译：

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基于海洋塑料碎片生物膜中占主导地位的降解和非降解细菌的高效聚苯乙烯降解微生物群体的构建


发泡聚苯乙烯 （EPS） 在海洋环境中造成了严重污染，在长期漂浮的 EPS 生物膜上发现了潜在的 EPS 降解细菌。然而，关于细菌相互作用和基于原位细菌多样性的联盟重建的研究仍然有限。从厦门岛亚热带海岸收集不同大小的海洋 EPS 废弃物，并进行细菌多样性分析。共现网络和细菌表征表明，红杆菌属和根瘤菌属在聚苯乙烯 （PS） 降解中起重要作用。细菌分离表征证实 Fulvimarina pelagi、Pseudosulfitobacter pseudonitzschiae、Devosia nitrariae、Cytobacillus kochii 和 Cytobacillus oceanisediminis 是新的 PS 降解剂。基于它们丰富的原位和 PS 降解活性，构建了一个由 F. pelagi、P. halotolerans 组成的联盟。和 O. granulosus 显示出高降解能力，PS 重量在 45 天内减轻了 18.9%。这些结果有助于海洋塑料污染修复和资源循环利用。