拉曼光谱揭示了对天然和组织工程关节软骨的区域组织的新见解。,ACS Central Science

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拉曼光谱揭示了对天然和组织工程关节软骨的区域组织的新见解。
ACS Central Science ( IF 12.7 ) Pub Date : 2016-11-16 , DOI: 10.1021/acscentsci.6b00222
Mads S Bergholt ₁ , Jean-Philippe St-Pierre ₁ , Giovanni S Offeddu ₂ , Paresh A Parmar ₁ , Michael B Albro ₁ , Jennifer L Puetzer ₁ , Michelle L Oyen ₂ , Molly M Stevens ₁

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组织结构与其功能密切相关，组织组织的丧失通常与病理有关。关节软骨中复杂的深度依赖性细胞外基质 (ECM) 排列对其生物力学功能至关重要。在这项研究中，我们开发了一种拉曼光谱成像方法，以使用牛组织和细胞获得对天然和组织工程关节软骨的深度依赖性排列的新见解。我们的结果基于主要 ECM 成分分布和方向的主成分分析和 k 均值聚类分析，揭示了以前未报告的组织复杂性在潮线上方至少六个区域。纳米压痕和拉曼光谱数据的相关性表明，整个组织深度的生物力学受 ECM 微观结构而不是成分的影响。此外，拉曼光谱与多变量分析一起揭示了组织工程构建物中胶原蛋白、糖胺聚糖和水的分布随时间的变化。这些变化是使用简单的指标进行评估的，这些指标有望指导具有天然区域复杂性和功能性能的广泛组织的再生。

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Raman Spectroscopy Reveals New Insights into the Zonal Organization of Native and Tissue-Engineered Articular Cartilage.

Tissue architecture is intimately linked with its functions, and loss of tissue organization is often associated with pathologies. The intricate depth-dependent extracellular matrix (ECM) arrangement in articular cartilage is critical to its biomechanical functions. In this study, we developed a Raman spectroscopic imaging approach to gain new insight into the depth-dependent arrangement of native and tissue-engineered articular cartilage using bovine tissues and cells. Our results revealed previously unreported tissue complexity into at least six zones above the tidemark based on a principal component analysis and k-means clustering analysis of the distribution and orientation of the main ECM components. Correlation of nanoindentation and Raman spectroscopic data suggested that the biomechanics across the tissue depth are influenced by ECM microstructure rather than composition. Further, Raman spectroscopy together with multivariate analysis revealed changes in the collagen, glycosaminoglycan, and water distributions in tissue-engineered constructs over time. These changes were assessed using simple metrics that promise to instruct efforts toward the regeneration of a broad range of tissues with native zonal complexity and functional performance.

更新日期：2016-11-16

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