配体-受体相互作用调节酶指导的小分子自组装的能量景观,Journal of the American Chemical Society

当前位置： X-MOL 学术 › J. Am. Chem. Soc. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

配体-受体相互作用调节酶指导的小分子自组装的能量景观
Journal of the American Chemical Society ( IF 14.4 ) Pub Date : 2016-11-15 , DOI: 10.1021/jacs.6b07677
Richard Haburcak ₁ , Junfeng Shi ₁ , Xuewen Du ₁ , Dan Yuan ₁ , Bing Xu ₁

Affiliation

酶促反应和配体-受体相互作用的同时发生对于蛋白质来说很常见，但对于小分子来说很少见，并且还有待探索。在这里，我们表明配体 - 受体相互作用调节由酶指导的小分子组装形成的分子组装的形态。虽然没有配体 - 受体相互作用允许前体的酶促脱磷酸产生自组装形成长纳米纤维的水凝胶剂，但配体 - 受体相互作用的存在偏向于形成包含短纳米纤维的陡峭聚集体的途径。当水凝胶剂自组装形成无法与配体（即万古霉素）结合的纳米纤维或纳米带时，表面活性剂的加入会破坏组装体以恢复配体-受体相互作用。此外，过量的配体会破坏纳米纤维并导致沉淀。作为使用配体 - 受体相互作用来调节酶自组装动力学的第一个例子，这项工作不仅提供了评估聚集体和目标分子之间相互作用的解决方案，而且还为理解复杂的突发行为提供了新的见解。具有多个平行过程的分子系统。

"点击查看英文标题和摘要"

Ligand–Receptor Interaction Modulates the Energy Landscape of Enzyme-Instructed Self-Assembly of Small Molecules

The concurrence of enzymatic reaction and ligand–receptor interactions is common for proteins, but rare for small molecules and has yet to be explored. Here we show that ligand–receptor interaction modulates the morphology of molecular assemblies formed by enzyme-instructed assembly of small molecules. While the absence of ligand–receptor interaction allows enzymatic dephosphorylation of a precursor to generate the hydrogelator that self-assembles to form long nanofibers, the presence of the ligand–receptor interaction biases the pathway to form precipitous aggregates containing short nanofibers. While the hydrogelators self-assemble to form nanofibers or nanoribbons that are unable to bind with the ligand (i.e., vancomycin), the addition of surfactant breaks up the assemblies to restore the ligand–receptor interaction. In addition, an excess amount of the ligands can disrupt the nanofibers and result in the precipitates. As the first example of the use of ligand–receptor interaction to modulate the kinetics of enzymatic self-assembly, this work not only provides a solution to evaluate the interaction between aggregates and target molecules but also offers new insight for understanding the emergent behavior of sophisticated molecular systems having multiple and parallel processes.

更新日期：2016-11-15

点击分享查看原文

点击收藏

公开下载

阅读更多本刊新发论文本刊介绍/投稿指南