Four novel metabolites, named isofusidienol A, B, C, and D (1–4), were produced by cultures of Chalara sp. (strain 6661), an endophytic fungus isolated from Artemisia vulgaris. The unprecedented chromone-3-oxepine structure of the compounds was established by detailed spectroscopic analysis and in the case of isofusidienol A (1) verified by an X-ray analysis. Additionally, two xanthones, known 5 and its 8-chloro derivative 6, were isolated. Presumably, 5 is the biosynthetic precursor of the isofusidienols. The isofusidienols exhibit antifungal activity against Candida albicans and antibacterial activity against gram-positive and gram-negative bacteria. Inhibition of Bacillus subtilis could be achieved with less than 0.625 μg of 1 on 6-mm filter disks in plate diffusion assays. (© Wiley-VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2008)

中文翻译：

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Isofusidienols：由内生真菌 Chalara sp. 产生的新型 Chromone-3-oxepines。

Chalara sp. 的培养物产生了四种新的代谢物，命名为异呋喃西林 A、B、C 和 D (1-4)。（菌株 6661），一种从 Artemisia vulgaris 中分离的内生真菌。通过详细的光谱分析和 X 射线分析验证了异呋喃烯醇 A (1) 的情况，建立了化合物前所未有的 chromone-3-oxepine 结构。此外，还分离出了两种已知的氧杂蒽酮 5 及其 8-氯衍生物 6。据推测，5 是异呋喃二烯醇的生物合成前体。异呋喃二烯醇对白色念珠菌具有抗真菌活性，对革兰氏阳性菌和革兰氏阴性菌具有抗菌活性。在平板扩散试验中，在 6 毫米滤盘上使用少于 0.625 μg 的 1 即可抑制枯草芽孢杆菌。(© Wiley-VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2008)