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Oxygen Sensing Difluoroboron β-Diketonate Polylactide Materials with Tunable Dynamic Ranges for Wound Imaging
ACS Sensors ( IF 8.2 ) Pub Date : 2016-11-14 00:00:00 , DOI: 10.1021/acssensors.6b00533 Christopher A. DeRosa 1 , Scott A. Seaman 2 , Alexander S. Mathew 1 , Catherine M. Gorick 2 , Ziyi Fan 1 , James N. Demas 1 , Shayn M. Peirce 2 , Cassandra L. Fraser 1, 2
ACS Sensors ( IF 8.2 ) Pub Date : 2016-11-14 00:00:00 , DOI: 10.1021/acssensors.6b00533 Christopher A. DeRosa 1 , Scott A. Seaman 2 , Alexander S. Mathew 1 , Catherine M. Gorick 2 , Ziyi Fan 1 , James N. Demas 1 , Shayn M. Peirce 2 , Cassandra L. Fraser 1, 2
Affiliation
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Difluoroboron β-diketonate poly(lactic acid) materials exhibit both fluorescence (F) and oxygen sensitive room-temperature phosphorescence (RTP). Introduction of halide heavy atoms (Br and I) is an effective strategy to control the oxygen sensitivity in these materials. A series of naphthyl-phenyl (nbm) dye derivatives with hydrogen, bromide, and iodide substituents were prepared for comparison. As nanoparticles, the hydrogen derivative was hypersensitive to oxygen (0–0.3%), while the bromide analogue was suited for hypoxia detection (0–3% O2). The iodo derivative, BF2nbm(I)PLA, showed excellent F to RTP peak separation and a 0–100% oxygen sensitivity range, unprecedented for metal-free RTP emitting materials. Due to the dual emission and exceptionally long RTP lifetimes of these O2 sensing materials, a portable, cost-effective camera was used to quantify oxygen levels via lifetime and red/green/blue (RGB) ratiometry. The hypersensitive H dye was well matched to lifetime detection; simultaneous lifetime and ratiometric imaging was possible for the bromide analogue, whereas the iodide material, with intense RTP emission and a shorter lifetime, was suited for RGB ratiometry. To demonstrate the prospects of this camera/material design combination for bioimaging, iodide boron dye-PLA nanoparticles were applied to a murine wound model to detect oxygen levels. Surprisingly, wound oxygen imaging was achieved without covering (i.e., without isolating from ambient conditions, air). Additionally, wound healing was monitored via wound size reduction and associated oxygen recovery, from hypoxic to normoxic. These single-component materials provide a simple tunable platform for biological oxygen sensing that can be deployed to spatially resolve oxygen in a variety of environments.
中文翻译：
具有可调动态范围的伤口成像的氧传感二氟硼β-二酮酸聚丙交酯材料
β-二酮硼酸二氟硼酸聚乳酸材料同时具有荧光（F）和氧敏感性室温磷光（RTP）。引入卤化物重原子（Br和I）是控制这些材料中氧敏感性的有效策略。制备了一系列具有氢，溴和碘取代基的萘基苯基（nbm）染料衍生物用于比较。作为纳米粒子，氢衍生物对氧气非常敏感（0–0.3％），而溴化物类似物则适合进行缺氧检测（0–3％O 2）。碘代衍生物BF 2 nbm（I）PLA表现出出色的F到RTP峰分离和0-100％的氧敏感性范围，这对于无金属的RTP发射材料来说是空前的。由于这些O的双重发射和极长的RTP寿命2个感应材料，便携式，高性价比的相机用于通过寿命和红/绿/蓝（RGB）比率测定法来量化氧气含量。超敏H染料与寿命检测非常匹配。溴化物类似物可以同时进行寿命和比例成像，而具有强烈RTP发射且寿命较短的碘化物材料适合RGB比例测量。为了证明这种相机/材料设计组合用于生物成像的前景，将碘化硼染料-PLA纳米颗粒应用于小鼠伤口模型以检测氧水平。令人惊讶地，在没有覆盖的情况下实现了伤口氧成像（即，没有与周围环境隔绝空气）。另外，通过伤口尺寸的减小和相关的氧气恢复（从低氧到常氧）来监测伤口的愈合。
更新日期：2016-11-14
中文翻译：
具有可调动态范围的伤口成像的氧传感二氟硼β-二酮酸聚丙交酯材料
β-二酮硼酸二氟硼酸聚乳酸材料同时具有荧光（F）和氧敏感性室温磷光（RTP）。引入卤化物重原子（Br和I）是控制这些材料中氧敏感性的有效策略。制备了一系列具有氢，溴和碘取代基的萘基苯基（nbm）染料衍生物用于比较。作为纳米粒子，氢衍生物对氧气非常敏感（0–0.3％），而溴化物类似物则适合进行缺氧检测（0–3％O 2）。碘代衍生物BF 2 nbm（I）PLA表现出出色的F到RTP峰分离和0-100％的氧敏感性范围，这对于无金属的RTP发射材料来说是空前的。由于这些O的双重发射和极长的RTP寿命2个感应材料，便携式，高性价比的相机用于通过寿命和红/绿/蓝（RGB）比率测定法来量化氧气含量。超敏H染料与寿命检测非常匹配。溴化物类似物可以同时进行寿命和比例成像，而具有强烈RTP发射且寿命较短的碘化物材料适合RGB比例测量。为了证明这种相机/材料设计组合用于生物成像的前景，将碘化硼染料-PLA纳米颗粒应用于小鼠伤口模型以检测氧水平。令人惊讶地，在没有覆盖的情况下实现了伤口氧成像（即，没有与周围环境隔绝空气）。另外，通过伤口尺寸的减小和相关的氧气恢复（从低氧到常氧）来监测伤口的愈合。
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