Ru Atoms and Human Hemoglobin Confined in Nanoporous ZIF-67 as a Peroxidase-Mimicking Nanozyme for Cancer Identification,ACS Applied Nano Materials

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Ru Atoms and Human Hemoglobin Confined in Nanoporous ZIF-67 as a Peroxidase-Mimicking Nanozyme for Cancer Identification
ACS Applied Nano Materials ( IF 5.3 ) Pub Date : 2025-02-11 , DOI: 10.1021/acsanm.4c06311
Qinfeng Zhang ₁ , Zhanglei Yang ₂ , Chuanmin Fan ₁ , Gao Liu ₁ , Fasheng Liu ₃ , Hongyuan Shang _{4,

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Affiliation

Precise diagnosis of a malignant tumor of a high degree is vital for highly effective prevention and the treatment of quality of human life, and abnormal levels of glucose or H₂O₂ have been regarded as potential biomarkers for their functions in cancer origin, progression, and occurrence of metastasis. The levels of glucose and H₂O₂ are remarkably different in cancer cells than in normal cells. Herein, an on-site colorimetric biosensor for cancer-related glucose and H₂O₂ is developed utilizing a porous ruthenium (Ru) and hemoglobin human (HSA)-doped imidazole-based metal–organic framework (Ru_0.13/HSA_0.06/ZIF-67) as an outstanding peroxidase-mimicking nanozyme. Specifically, Ru_0.13/HSA_0.06/ZIF-67 nanohybrids are prepared by in situ doping of HSA into ZIF-67 followed by an ion-exchange process. Taking advantage of the unique structures of natural enzymes and interface engineering, HSA and Ru are confined in continuous porous ZIF-67 to largely expose the active sites for the created nanopores. The obtained distinct structure considerably boosts peroxidase-mimicking activity. The colorimetric assay reveals that the peroxidase-mimicking capability of the Ru_0.13/HSA_0.06/ZIF-67 nanohybrid is superior to that of Fe-based natural enzymes and other Ru catalysts. Moreover, a Ru_0.13/HSA_0.06/ZIF-67 nanozyme-based colorimetric assay is constructed for detecting glucose and H₂O₂ via a visible signal readout. The developed toolkit for the preparation of Ru_0.13/HSA_0.06/ZIF-67 nanozymes with peroxidase-mimicking performance offers an avenue for rationally designing distinct nanozymes and has the potential perspectives for clinical transformation in colorimetric analysis.

中文翻译：

Ru 原子和人血红蛋白局限在纳米多孔 ZIF-67 中，作为过氧化物酶模拟纳米酶用于癌症鉴定

高度恶性肿瘤的精确诊断对于人类生活质量的高效预防和治疗至关重要，葡萄糖或 H ₂ O ₂ 水平异常被认为是它们在癌症发生、进展和转移发生中发挥作用的潜在生物标志物。癌细胞中的葡萄糖和 H ₂ O ₂ 水平与正常细胞明显不同。在此，利用多孔钌（Ru）和血红蛋白（HSA）掺杂咪唑基金属有机框架（Ru _0.13 /HSA _0.06 /ZIF-67）作为出色的过氧化物酶模拟纳米酶，开发了一种用于癌症相关葡萄糖和 H ₂ O ₂ 的现场比色生物传感器。具体来说，Ru _0.13 /HSA _0.06 /ZIF-67 纳米杂化物是通过 HSA 原位掺杂到 ZIF-67 中，然后进行离子交换过程制备的。利用天然酶和界面工程的独特结构，HSA 和 Ru 被限制在连续多孔 ZIF-67 中，以在很大程度上暴露所创建纳米孔的活性位点。获得的独特结构大大提高了过氧化物酶模拟活性。比色测定表明，Ru _0.13 /HSA _0.06 /ZIF-67 纳米杂化物的过氧化物酶模拟能力优于基于 Fe 的天然酶和其他 Ru 催化剂。此外，构建了基于 Ru _0.13 /HSA _0.06 /ZIF-67 纳米酶的比色测定法， ₂ 用于通过可见信号读数检测葡萄糖和 HO ₂ 。开发的用于制备具有过氧化物酶模拟性能的 Ru _0.13 /HSA _0.06 /ZIF-67 纳米酶的工具包为合理设计不同的纳米酶提供了一条途径，并具有比色分析临床转化的潜在前景。

更新日期：2025-02-11

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