通过批处理和XAFS技术研究<i>米根霉</i>中As（V）的固定化,ACS Omega

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通过批处理和XAFS技术研究米根霉中As（V）的固定化
ACS Omega ( IF 3.7 ) Pub Date : 2016-11-11 00:00:00 , DOI: 10.1021/acsomega.6b00260
Wencheng Song ₁ , Xiangxue Wang ₁ , Tao Wen ₁ , Shujun Yu ₁ , Yidong Zou ₁ , Yubing Sun , Tasawar Hayat ₂ , Xiangke Wang _{1,

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Affiliation

由于对人体健康的巨大危害，水溶液中的砷（As）污染已引起越来越多的公众关注。本文中，在不同的环境条件下，例如不同的pH，离子强度，菌丝体剂量，菌丝体生长和温度，研究了米根霉在水系统中对砷酸盐（As（V））的生物富集。结果表明，米（R. oryzae）可以有效地富集砷（V），在T = 299 K时，米（R. oryzae）中的最大砷（V）生物蓄积量为52.4 mg / g，远高于其他稻。相似条件下的生物材料。米曲霉在As（V）胁迫下生成更高含量的硫醇化合物，以固定水溶液中的As（V）。X射线吸收近缘光谱分析表明，随着接触时间的延长，As（V）部分还原为As（III），这增加了菌丝中As（V）的生物积累。此外，扩展的X射线吸收精细结构分析表明，As-S络合物在菌丝体固定As（V）中起着重要作用。这项研究提供了深入的研究，在分子尺度上对米根霉细胞内As的形态和配位关系，这对于了解环境清洁过程中As（V）与真菌的相互作用机制至关重要。

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Immobilization of As(V) in Rhizopus oryzae Investigated by Batch and XAFS Techniques

Arsenic (As) contamination in aqueous solutions has become an increasing public concern due to the immense harm to human health. Herein, bioaccumulation of arsenate (As(V)) by Rhizopus oryzae in aqueous systems was investigated under different environmental conditions, such as different pH’s, ionic strengths, mycelia dosages, mycelia growths, and temperatures. The results showed that As(V) could be bioaccumulated efficiently by R. oryzae, and the maximum bioaccumulation capacity of As(V) in R. oryzae was 52.4 mg/g at T = 299 K, which was much higher than that for other biomaterials under similar conditions. R. oryzae generated a higher content of thiol compounds under As(V) stress to immobilize As(V) from aqueous solutions. X-ray absorption near-edge spectroscopy analysis indicated that As(V) was partly reduced to As(III) with increasing contact time, which increased As(V) bioaccumulation in mycelia. In addition, extended X-ray absorption fine structure analysis showed that the As–S complex played an important role in As(V) immobilization by mycelia. This study provided an in-depth investigation of intracellular As speciation and coordination in R. oryzae on the molecular scale, which was crucial to understand the interaction mechanisms of As(V) with fungi during environmental cleanup.

更新日期：2016-11-11

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