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Recent advances of fluorescent probe for the conjugated enzymes closely related to clinical diseases
Coordination Chemistry Reviews ( IF 20.3 ) Pub Date : 2025-01-28 , DOI: 10.1016/j.ccr.2025.216471
Ren-Wei-Yang Zhang, Han Zhang, Qiu Li, Fa-Ping Li, Rong-Ya Zhang, Wei-Bo Zhao, Hong-Ji Liu, Zhi-Gang Hu, Kai Wang

The four biological enzymes encompassing alkaline phosphatase (ALP), aminopeptidase N (APN), γ-glutamyl transferase (GGT), and Cathepsin B (CTB), play a pivotal role in a myriad of physiological and pathological processes. Hence, the detection of the five indexes could own an important value in the diagnosis of clinical diseases even the subsequent treatments, which is restricted by a traditional method circle mainly consisting of liquid chromatography, gas chromatography, inductively coupled plasma-atomic emission spectrometry, chemiluminescence, and inductively coupled plasma-atomic emission spectrometry with several defaults such as bulky equipment, intricate process of testing, elevated detection expenses, high costs, and the lack of visualization in living organisms. The introduction of fluorescent probes brings a novel perspective to sense the biochemical indicators with a rapid non-invasive effect accompanied by high accuracy and sensitivity in both physiological and pathological contexts. This review introduced the fluorescence probes for these biochemical molecules (ALP, APN, GGT, and CTB) by their optical imaging in vitro assay with the models of various cell lines (HeLa, A549, HepG2, etc.) and wine, the imaging in vivo assay by the models of animals (Mice, zebrafishes, etc.) even human samples as well from 2014 to 2024. Furthermore, we systematically illustrate the optimistic progress of the fluorescent probes targeting the five molecules and expect the bright prospects of the fluorescence chemosensors shortly with a sincere hope of benefit for subsequent studies in the biological processes pivotal for related clinical application.

中文翻译:


与临床疾病密切相关的偶联酶荧光探针的研究进展



包括碱性磷酸酶 (ALP)、氨肽酶 N (APN)、γ-谷氨酰转移酶 (GGT) 和组织蛋白酶 B (CTB) 在内的四种生物酶在无数的生理和病理过程中起着关键作用。因此,这五项指标的检测在临床疾病的诊断甚至后续治疗中都具有重要价值,这受到主要由液相色谱法、气相色谱法、电感耦合等离子体原子发射光谱法、化学发光法和电感耦合等离子体原子发射光谱法组成的传统方法圈的限制,具有一些默认条件,例如设备笨重, 检测过程复杂、检测费用高、成本高以及生物体中缺乏可视化。荧光探针的引入为感知生化指标带来了一种新的视角,在生理和病理环境中具有快速的非侵入性效果,同时具有很高的准确性和灵敏度。本文介绍了这些生化分子 (ALP、APN、GGT 和 CTB) 的荧光探针,通过光学成像体外测定各种细胞系 (HeLa、A549、HepG2 等) 和葡萄酒,动物 (小鼠、斑马鱼等) 模型甚至人类样本的成像体内测定以及 2014 年至 2024 年。此外,我们系统地说明了靶向 5 个分子的荧光探针的乐观进展,并期待荧光化学传感器的光明前景,并真诚地希望为相关临床应用关键的生物过程的后续研究带来益处。
更新日期:2025-01-28
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