当前位置:
X-MOL 学术
›
Pest Manag. Sci.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Increase in IAA levels by EPSPS copy number variation relates to fitness advantage in Eleusine indica
Pest Management Science ( IF 3.8 ) Pub Date : 2025-01-27 , DOI: 10.1002/ps.8637
Jingchao Chen, Hailan Cui, Zhiling Li, Haiyan Yu, Qin Yu, Xiangju Li
Pest Management Science ( IF 3.8 ) Pub Date : 2025-01-27 , DOI: 10.1002/ps.8637
Jingchao Chen, Hailan Cui, Zhiling Li, Haiyan Yu, Qin Yu, Xiangju Li
BACKGROUNDLong‐term use of chemical weed control has led to some weedy species evolving herbicide resistance traits with fitness advantage. Our previous studies revealed glyphosate resistance in an Eleusine indica population due to copy number variation of 5‐enolpyruvylshikimate‐3‐phosphate synthase (EPSPS ) comes with fitness advantage under non‐competitive conditions. Here, transcriptomics and targeted metabolomics were used to investigate physiological basis associated with the fitness advantage.RESULTSRelative copy number of EPSPS gene and plant dry weight of the glyphosate‐resistant (R) population was 88.3‐ and 1.2‐ times, respectively, higher than that in the wild type (WT) plants that were isolated from within the R population. Seven genes were screened to be relevant to fitness growth trait by RNA‐seq. The level of aromatic amino acids Tryptophan (Trp), Phenylalanine (Phe) and Tyrosine (Tyr), products in the shikimate pathway catalyzed by EPSPS, was 1.2‐times higher in R compared to the WT plants. The metabolites associated with Trp metabolism indole‐3‐acetic acid (IAA), 3‐indolepropionic acid (IPA), indole‐3‐acetamide (IAM) in the R plants were 2.0‐, 1.8‐ and 1.4‐ times higher than that in the WT plants, respectively.CONCLUSIONAll the results indicate that fitness advantage in the studied R E. indica population may be caused by higher IAA production due to over‐expression of the EPSPS gene and pleiotropically by elevated carbon metabolism. The findings in this research can provide reference information for control strategies to the glyphosate‐resistant E. indica . © 2025 Society of Chemical Industry.
中文翻译:
EPSPS 拷贝数变异导致 IAA 水平的增加与刺五花的适应度优势有关
背景长期使用化学杂草控制导致一些杂草物种进化出具有适应性优势的除草剂抗性特征。我们之前的研究揭示了由于 5-烯醇丙酮基莽草酸盐-3-磷酸合酶 (EPSPS) 的拷贝数变化,Eleusine indica 种群中的草甘膦耐药性在非竞争条件下具有适应性优势。在这里,转录组学和靶向代谢组学用于研究与健身优势相关的生理基础。结果抗草甘膦 (R) 种群的 EPSPS 基因拷贝数和植株干重分别是从 R 种群中分离的野生型 (WT) 植株的 88.3 倍和 1.2 倍。通过 RNA-seq 筛选出 7 个与健身生长性状相关的基因。EPSPS 催化的莽草酸盐途径中的芳香族氨基酸色氨酸 (Trp)、苯丙氨酸 (Phe) 和酪氨酸 (Tyr) 的水平在 R 中比 WT 植物高 1.2 倍。R 植物中与 Trp 代谢相关的代谢物吲哚-3-乙酸 (IAA)、3-吲哚丙酸 (IPA)、吲哚-3-乙酰胺 (IAM) 分别比 WT 植物高 2.0 倍、1.8 倍和 1.4 倍。结论所有结果表明,所研究的 R E. indica 种群的适应性优势可能是由于 EPSPS 基因的过度表达导致 IAA 产生增加,以及碳代谢升高导致多效性增加。本研究结果可为抗草甘膦 E. indica 的防治策略提供参考信息。© 2025 化工学会.
更新日期:2025-01-27
中文翻译:
EPSPS 拷贝数变异导致 IAA 水平的增加与刺五花的适应度优势有关
背景长期使用化学杂草控制导致一些杂草物种进化出具有适应性优势的除草剂抗性特征。我们之前的研究揭示了由于 5-烯醇丙酮基莽草酸盐-3-磷酸合酶 (EPSPS) 的拷贝数变化,Eleusine indica 种群中的草甘膦耐药性在非竞争条件下具有适应性优势。在这里,转录组学和靶向代谢组学用于研究与健身优势相关的生理基础。结果抗草甘膦 (R) 种群的 EPSPS 基因拷贝数和植株干重分别是从 R 种群中分离的野生型 (WT) 植株的 88.3 倍和 1.2 倍。通过 RNA-seq 筛选出 7 个与健身生长性状相关的基因。EPSPS 催化的莽草酸盐途径中的芳香族氨基酸色氨酸 (Trp)、苯丙氨酸 (Phe) 和酪氨酸 (Tyr) 的水平在 R 中比 WT 植物高 1.2 倍。R 植物中与 Trp 代谢相关的代谢物吲哚-3-乙酸 (IAA)、3-吲哚丙酸 (IPA)、吲哚-3-乙酰胺 (IAM) 分别比 WT 植物高 2.0 倍、1.8 倍和 1.4 倍。结论所有结果表明,所研究的 R E. indica 种群的适应性优势可能是由于 EPSPS 基因的过度表达导致 IAA 产生增加,以及碳代谢升高导致多效性增加。本研究结果可为抗草甘膦 E. indica 的防治策略提供参考信息。© 2025 化工学会.