<br>纳米封装光纤基 PEC 微电极：NT-proBNP 及其植入性能的高灵敏度和特异性检测,Analytical Chemistry

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纳米封装光纤基 PEC 微电极：NT-proBNP 及其植入性能的高灵敏度和特异性检测
Analytical Chemistry ( IF 6.7 ) Pub Date : 2025-01-27 , DOI: 10.1021/acs.analchem.4c04757
HuiYu Zou ₁ , Zhizhong Jiang ₂ , ErKang Bian ₁ , Jingjing Zhou ₃ , Shengqing Li ₂ , Yicheng Yang ₄ , HuiPing Guo ₁ , Yuancheng Liu ₅ , WuMing Wu ₂ , ChunYan Deng ₁

Affiliation

微电极具有出色的灵敏度、快速响应和多功能性，使其成为实时检测和监测应用的理想选择。光电化学（PEC）传感器因其高灵敏度、快速响应和易于作而在许多领域显示出巨大的价值。然而，传统的 PEC 传感依赖于笨重的外部光源和笨重的电极，阻碍了其小型化和植入，从而限制了其在实时疾病监测中的应用。为了克服这些限制，我们开发了一种基于纳米封装光纤（OF）的 PEC 微电极。微电极具有 TiO₂/CdS 纳米晶体和双（2,2′-联吡啶）（10-甲基菲咯啉 [3,2-a：2′3′-c] 吡啶钌（II）二氯化物（[Ru（bpy）₂dppz]²⁺） @dsDNA/Au@epigallocatechin 没食子酸盐纳米颗粒（EGCG NP）层。并探讨了其在检测 N 末端脑利钠肽前体（NT-proBNP）作为心血管疾病生物标志物的应用。实现了 1–5000 pg mL^–1 的宽线性范围和 0.36 pg mL^–1 的低检测限。该范围不仅涵盖在所有年龄组个体的临床诊断中排除心血管疾病的推荐阈值，还涵盖预后目标值。该传感器在血清检测中表现出优异的选择性和稳定性以及显著的标记恢复能力。至关重要的是，该传感器成功区分了人平滑肌细胞内 NT-proBNP 分泌水平的变化，比较了血小板衍生生长因子-BB 的刺激前后。更重要的是，在小鼠身上进行的皮肤穿刺实验证明了 OF-PEC 微电极显着的植入性和生物相容性。这解决了微电极在用作植入式设备时通常面临的关键挑战，例如最大限度地减少侵入性创伤、减轻炎症和防止生物污染，从而牢固地确立了它们适合开发先进的植入式传感设备。因此，目前的 OF 微电极 PEC 生物传感器不仅具有成本效益、易于作和小型化，而且在实现更精确、更微创和连续监测生物标志物而不会引起炎症方面具有巨大潜力。这种能力对于早期疾病检测、跟踪疾病进展和促进个性化治疗策略至关重要，这扩大了 PEC 传感器的实际应用。

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Nanoencapsulated Optical Fiber-Based PEC Microelectrode: Highly Sensitive and Specific Detection of NT-proBNP and Its Implantable Performance

Microelectrodes offer exceptional sensitivity, rapid response, and versatility, making them ideal for real-time detection and monitoring applications. Photoelectrochemical (PEC) sensors have shown great value in many fields due to their high sensitivity, fast response, and ease of operation. Nevertheless, conventional PEC sensing relies on cumbersome external light sources and bulky electrodes, hindering its miniaturization and implantation, thereby limiting its application in real-time disease monitoring. To overcome these limitations, we developed a nanoencapsulated optical fiber (OF)-based PEC microelectrode. The microelectrode features TiO₂/CdS nanocrystals and bis (2,2′-bipyridine) (10-methylphenanthroline [3,2-a:2′3′-c] pyridine ruthenium(II) dichloride ([Ru(bpy)₂dppz]²⁺) @dsDNA/Au@epigallocatechin gallate nanoparticle (EGCG NP) layers. And its application for the detection of N-terminal pro-brain natriuretic peptide (NT-proBNP) as a biomarker of cardiovascular diseases was explored. An extensive linear range of 1–5000 pg mL^–1 combined with a low detection limit of 0.36 pg mL^–1 was achieved. This range covers not only the recommended threshold for excluding cardiovascular diseases in the clinical diagnosis of individuals across all age groups but also the prognostic target value. The sensor exhibited excellent selectivity and stability and notable labeling recovery capability in serum tests. Critically, the sensor successfully discriminated the alterations in NT-proBNP secretion levels within human smooth muscle cells, comparing pre- and poststimulation by platelet-derived growth factor-BB. Even more significantly, the skin puncture experiment conducted in mice demonstrated the remarkable implantability and biological compatibility of the OF-PEC microelectrode. This addresses critical challenges commonly faced by microelectrodes when used as implanted devices, such as minimizing invasive trauma, mitigating inflammation, and preventing biofouling, thereby firmly establishing their suitability for the development of advanced implantable sensing devices. Therefore, the present OF microelectrode PEC biosensor is not only cost-effective, easy to operate, and miniaturized but also holds significant potential for enabling more precise, more minimally invasive, and continuous monitoring of biological markers without causing inflammation. This capability is crucial for early disease detection, tracking disease progression, and facilitating personalized treatment strategies, which expands the practical application of PEC sensors.

更新日期：2025-01-27

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