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Biological VFAs production from proteinaceous wastewater varied with protein type: The role of protein exposed enzyme cleavage sites and hydrolysates biotransformation capacity
Water Research ( IF 11.4 ) Pub Date : 2025-01-25 , DOI: 10.1016/j.watres.2025.123201
Jing Zhou, Dapeng Li, Xu Duan, Xuemeng Zhang, Chuang Chen, Yinguang Chen

Proteinaceous wastewater contains various proteins, which can be valorized to biobased volatile fatty acids (VFAs), important substrates for the synthesis of biodegradable plastics, biodiesel, bioelectricity, etc., but the influence of protein type on VFAs has never been documented. It was found that among the five proteinaceous wastewater proteins investigated, ovalbumin and casein produced the most and the least VFAs, respectively. The mechanism investigation shows that proteins with higher VFAs production had higher functional microorganism abundance and key enzyme activity in the reaction system due to their more enzyme cleavage sites and looser secondary structure, which made it easier for more hydrolase to bind, causing more protein hydrolysis. Also, metaproteomics and amino acid composition analyses revealed that the hydrolysates of proteins with higher VFAs had more isoleucine and proline, which were needed for the synthesis of recognizing and binding proteins (oligopeptide permease subunit A (OppA) and dipeptide permease (Dpp)) of acidogens and beneficial for transporters (Dpp subunit F/Opp subunit F), more hydrolysates (amino acids) were therefore transported into the cell. Further investigation indicates that more electron acceptor and electron donor paired amino acids were in the hydrolysates, facilitating the Stickland reaction and promoting intracellular amino acids bio-transformation to VFAs.

中文翻译:


蛋白质废水中生物 VFA 的生产因蛋白质类型而异:蛋白质暴露的酶切割位点和水解产物生物转化能力的作用



蛋白质废水中含有多种蛋白质,可转化为生物基挥发性脂肪酸 (VFA),这是合成可生物降解塑料、生物柴油、生物电等的重要底物,但蛋白质类型对 VFA 的影响从未被记录下来。结果发现,在研究的 5 种蛋白质废水蛋白中,卵清蛋白和酪蛋白分别产生最多和最少的 VFA。机制研究表明,VFAs 产量较高的蛋白质由于其更多的酶切割位点和更松散的二级结构,在反应系统中具有更高的功能微生物丰度和关键酶活性,这使得更多的水解酶更容易结合,导致更多的蛋白质水解。此外,宏蛋白质组学和氨基酸组成分析表明,具有较高 VFA 的蛋白质的水解物具有更多的异亮氨酸和脯氨酸,这是合成识别和结合酸原蛋白质 (寡肽通透酶亚基 A (OppA) 和二肽通透酶 (Dpp) 所必需的),并且对转运蛋白 (Dpp 亚基 F/Opp 亚基 F) 有益),因此更多的水解物 (氨基酸) 被转运到细胞中。进一步的研究表明,水解物中含有更多的电子受体和电子供体配对氨基酸,促进了 Stickland 反应并促进细胞内氨基酸向 VFA 的生物转化。
更新日期:2025-01-29
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