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Foliar spray double‐stranded RNA targeting HvIAP1 induces high larval and adult mortality in Henosepilachna vigintioctopunctata
Pest Management Science ( IF 3.8 ) Pub Date : 2025-01-25 , DOI: 10.1002/ps.8667
Tianliang Ji, Langcheng Li, Xin Zhu, Guirong Wang
Pest Management Science ( IF 3.8 ) Pub Date : 2025-01-25 , DOI: 10.1002/ps.8667
Tianliang Ji, Langcheng Li, Xin Zhu, Guirong Wang
BACKGROUNDExogenous double‐stranded RNA (dsRNA) has the potential to serve as an effective alternative to conventional chemical pesticides for the control of insect pests, because it can specifically inhibit essential gene expression in these organisms. However, identifying suitable gene targets remains a crucial step in the development of RNA interference (RNAi)‐based pest control strategies.RESULTSIn this study, three apoptosis‐related genes were selected to evaluate their potential for RNAi‐induced lethality in Henosepilachna vigintioctopunctata via foliar spray dsRNAs. Our results revealed that silencing of HvIAP1 (inhibitor of apoptosis protein 1) had a significant lethal effect on larvae, whereas silencing of HvAIF1 and HvASPP1 did not, correlating with distinct expression patterns. Larvae ingested leaves sprayed with bacterially expressed dsHvIAP1 at a concentration of 100 ng/cm2 resulting in the impairment of cuticle tanning, cessation of feeding, and ultimately, 100% mortality within 7 days. In addition, the potential of dsHvIAP1 to mediate for adult control was also investigated and showed that administration of 200 ng/cm2 dsHvIAP1 resulted in 100% adult mortality within 9 days, accompanied by a significant reduction in leaf consumption and suppression of HvIAP1 expression compared with the dsGFP group.CONCLUSIONSOur findings demonstrate that HvIAP1 is a highly lethal molecular target with a notable difference in the concentration required for larval and adult mortality. These findings provide a foundation for the future development of RNAi pesticides for environmentally friendly control of H. vigintioctopunctata . © 2025 Society of Chemical Industry.
中文翻译:
靶向 HvIAP1 的叶面喷施双链 RNA 诱导 Henosepilachna vigintioctopunctata 的幼虫和成虫死亡率高
背景异源性双链 RNA (dsRNA) 有可能成为传统化学杀虫剂控制害虫的有效替代品,因为它可以特异性抑制这些生物体中的基本基因表达。然而,确定合适的基因靶标仍然是开发基于 RNA 干扰 (RNAi) 的害虫防治策略的关键步骤。结果在本研究中,选择了 3 个细胞凋亡相关基因,以评估它们通过叶面喷施 dsRNAs 对 Henosepilachna vigintioctopunctata 的 RNAi 诱导致死的潜力。我们的结果显示,沉默 HvIAP1 (细胞凋亡蛋白 1 抑制剂) 对幼虫有显着的致死作用,而沉默 HvAIF1 和 HvASPP1 则没有,与不同的表达模式相关。幼虫摄入的叶子喷洒了浓度为 100 ng/cm2 的细菌表达的 dsHvIAP1,导致角质层晒黑受损,停止摄食,最终在 7 天内死亡率达到 100%。此外,还研究了 dsHvIAP1 介导成虫控制的潜力,结果表明,施用 200 ng/cm2 dsHvIAP1 导致 9 天内 100% 的成虫死亡率,同时与 dsGFP 组相比,叶片消耗显着减少并抑制 HvIAP1 表达。结论研究结果表明,HvIAP1 是一种高度致死的分子靶点,幼虫和成虫死亡率所需的浓度存在显着差异。这些发现为未来开发用于环境友好型防治 H. vigintioctopunctata 的 RNAi 农药提供了基础。© 2025 化工学会.
更新日期:2025-01-25
中文翻译:
靶向 HvIAP1 的叶面喷施双链 RNA 诱导 Henosepilachna vigintioctopunctata 的幼虫和成虫死亡率高
背景异源性双链 RNA (dsRNA) 有可能成为传统化学杀虫剂控制害虫的有效替代品,因为它可以特异性抑制这些生物体中的基本基因表达。然而,确定合适的基因靶标仍然是开发基于 RNA 干扰 (RNAi) 的害虫防治策略的关键步骤。结果在本研究中,选择了 3 个细胞凋亡相关基因,以评估它们通过叶面喷施 dsRNAs 对 Henosepilachna vigintioctopunctata 的 RNAi 诱导致死的潜力。我们的结果显示,沉默 HvIAP1 (细胞凋亡蛋白 1 抑制剂) 对幼虫有显着的致死作用,而沉默 HvAIF1 和 HvASPP1 则没有,与不同的表达模式相关。幼虫摄入的叶子喷洒了浓度为 100 ng/cm2 的细菌表达的 dsHvIAP1,导致角质层晒黑受损,停止摄食,最终在 7 天内死亡率达到 100%。此外,还研究了 dsHvIAP1 介导成虫控制的潜力,结果表明,施用 200 ng/cm2 dsHvIAP1 导致 9 天内 100% 的成虫死亡率,同时与 dsGFP 组相比,叶片消耗显着减少并抑制 HvIAP1 表达。结论研究结果表明,HvIAP1 是一种高度致死的分子靶点,幼虫和成虫死亡率所需的浓度存在显着差异。这些发现为未来开发用于环境友好型防治 H. vigintioctopunctata 的 RNAi 农药提供了基础。© 2025 化工学会.