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Nocturnin promotes NADH and ATP production for juvenile hormone biosynthesis in adult insects
Pest Management Science ( IF 3.8 ) Pub Date : 2025-01-25 , DOI: 10.1002/ps.8676
Lintao Qiao, Zitong Zhuang, Yage Wang, Kairui Xie, Xinyan Zhang, Yifan Shen, Jiasheng Song, Shutang Zhou
Pest Management Science ( IF 3.8 ) Pub Date : 2025-01-25 , DOI: 10.1002/ps.8676
Lintao Qiao, Zitong Zhuang, Yage Wang, Kairui Xie, Xinyan Zhang, Yifan Shen, Jiasheng Song, Shutang Zhou
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BACKGROUNDJuvenile hormone (JH) is a key endocrine governing insect development, metamorphosis and reproduction. JH analogs have offered great potential for insect pest control. In adulthood, JH titer rapidly increases in the previtellogenic period and reaches a peak in the vitellogenic phase. However, the regulatory mechanisms of JH biosynthesis in corpora allata (CA) of adult insects remain largely unknown.RESULTSWe observed that the mitochondrial abundance, as well as the levels of NADH (nicotinamide adenine dinucleotide, reduced form) and adenosine triphosphate (ATP), increased in the CA of previtellogenic adults, peaking during the vitellogenic stage. The transcripts of Nocturnin (Noct), which converts nicotinamide adenine dinucleotide phosphate (NADPH) to NADH for ATP production, were more abundant in the CA compared to those of other enzymes involved in conventional NADH‐producing metabolic pathways. The developmental expression pattern of Noct was like that of ATP and NADH level. RNA interference‐mediated knockdown of Noct caused a significant decrease of NADH and ATP contents, along with markedly reduced expression levels of 12 genes involved in JH biosynthesis pathway. Loss of Noct function resulted in remarkably reduced expression of vitellogenin , accompanied by arrested ovarian growth and oocyte maturation.CONCLUSIONOur results demonstrated that Noct plays a crucial role in high levels of JH biosynthesis in adult insects via regulating NADH and ATP production. The findings reveal a previously unknown aspect of mitochondrial metabolism in JH biosynthesis and provide valuable information for developing pest control strategies targeting hormone pathways. © 2025 Society of Chemical Industry.
中文翻译:
夜曲素促进 NADH 和 ATP 的产生,以促进成虫体内的幼年激素生物合成
背景黄蜂激素 (JH) 是控制昆虫发育、和繁殖的关键内分泌物质。JH 类似物为害虫防治提供了巨大的潜力。在成年期,JH 滴度在玻璃体生成前期迅速增加,并在玻璃体生成期达到峰值。然而,成虫 corpora allata (CA) 中 JH 生物合成的调节机制在很大程度上仍然未知。结果我们观察到,线粒体丰度以及 NADH (烟酰胺腺嘌呤二核苷酸,还原形式) 和三磷酸腺苷 (ATP) 的水平在 CA 中增加,在卵黄形成阶段达到峰值。夜曲素 (Noct) 的转录物将烟酰胺腺嘌呤二核苷酸磷酸 (NADPH) 转化为 NADH 以产生 ATP,与参与常规 NADH 产生代谢途径的其他酶的转录物相比,在 CA 中更丰富。Noct 的发育表达模式类似于 ATP 和 NADH 水平。RNA 干扰介导的 Noct 敲低导致 NADH 和 ATP 含量显著降低,同时参与 JH 生物合成途径的 12 个基因的表达水平显著降低。Noct 功能丧失导致卵黄素原蛋白表达显著降低,伴有卵巢生长停滞和卵母细胞成熟。结论研究结果表明,Noct 通过调节 NADH 和 ATP 的产生,在成虫高水平的 JH 生物合成中起关键作用。这些发现揭示了 JH 生物合成中线粒体代谢以前未知的方面,并为开发针对激素途径的害虫防治策略提供了有价值的信息。© 2025 化工学会.
更新日期:2025-01-25
中文翻译:
![](https://scdn.x-mol.com/jcss/images/paperTranslation.png)
夜曲素促进 NADH 和 ATP 的产生,以促进成虫体内的幼年激素生物合成
背景黄蜂激素 (JH) 是控制昆虫发育、和繁殖的关键内分泌物质。JH 类似物为害虫防治提供了巨大的潜力。在成年期,JH 滴度在玻璃体生成前期迅速增加,并在玻璃体生成期达到峰值。然而,成虫 corpora allata (CA) 中 JH 生物合成的调节机制在很大程度上仍然未知。结果我们观察到,线粒体丰度以及 NADH (烟酰胺腺嘌呤二核苷酸,还原形式) 和三磷酸腺苷 (ATP) 的水平在 CA 中增加,在卵黄形成阶段达到峰值。夜曲素 (Noct) 的转录物将烟酰胺腺嘌呤二核苷酸磷酸 (NADPH) 转化为 NADH 以产生 ATP,与参与常规 NADH 产生代谢途径的其他酶的转录物相比,在 CA 中更丰富。Noct 的发育表达模式类似于 ATP 和 NADH 水平。RNA 干扰介导的 Noct 敲低导致 NADH 和 ATP 含量显著降低,同时参与 JH 生物合成途径的 12 个基因的表达水平显著降低。Noct 功能丧失导致卵黄素原蛋白表达显著降低,伴有卵巢生长停滞和卵母细胞成熟。结论研究结果表明,Noct 通过调节 NADH 和 ATP 的产生,在成虫高水平的 JH 生物合成中起关键作用。这些发现揭示了 JH 生物合成中线粒体代谢以前未知的方面,并为开发针对激素途径的害虫防治策略提供了有价值的信息。© 2025 化工学会.