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Transcription factor BbCDR1 regulates the orchestration between conidial formation and maturation in the entomopathogenic fungus Beauveria bassiana
Pest Management Science ( IF 3.8 ) Pub Date : 2025-01-24 , DOI: 10.1002/ps.8662
Hui Zhang, Ajing Mao, Yu Liu, Yu Fu, Jiahao Cheng, Dan Jin, Yanhua Fan
Pest Management Science ( IF 3.8 ) Pub Date : 2025-01-24 , DOI: 10.1002/ps.8662
Hui Zhang, Ajing Mao, Yu Liu, Yu Fu, Jiahao Cheng, Dan Jin, Yanhua Fan
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BACKGROUNDThe entomopathogenic fungus Beauveria bassiana has been widely used for pest biocontrol with conidia serving as the main active agents. Conidial yield and quality are two important characteristics in fungal conidia development, however, the regulatory mechanisms that orchestrate conidial formation and development are not well understood.RESULTSIn this study, we identified a Zn2 Cys6 transcription factor BbCDR1 that inhibits conidial production while promoting conidial maturation. Compared with the wild type, the ΔBbCDR1 mutant exhibited a 1.88‐fold increase in conidial yield but a reduction in conidial quality, including decreased cell wall integrity and trehalose synthesis. The deletion of BbCDR1 also led to reduced conidial germination rates under oxidative, osmotic, conidial wall disruption and UV stresses. Interestingly, ΔBbCDR1 exhibited an increase of fungal virulence with a 12.5% decrease in LT50 compared to the wild type.CONCLUSIONSFurther analysis revealed that BbCDR1 represses the expression of BbbrlA and activates BbwetA , two components of the central developmental pathway (CDP) that regulate conidial initiation and maturation, respectively. These findings suggest that BbCDR1 plays a crucial role in conidial development and a new target gene for the genetic engineering of highly active fungal insecticides. © 2025 Society of Chemical Industry.
中文翻译:
转录因子 BbCDR1 调节昆虫病原真菌 Beauveria bassiana 分生孢子形成和成熟之间的协调
背景昆虫病原真菌 Beauveria bassiana 已被广泛用于害虫生物防治,其中分生孢子是主要的活性剂。分生孢子产量和质量是真菌分生孢子发育中的两个重要特征,然而,协调分生孢子形成和发育的调节机制尚不清楚。结果在本研究中,我们鉴定了一种 Zn2Cys6 转录因子 BbCDR1,该转录因子在促进分生孢子成熟的同时抑制分生孢子的产生。与野生型相比,ΔBbCDR1 突变体的分生孢子产量增加了 1.88 倍,但分生孢子质量降低,包括细胞壁完整性和海藻糖合成降低。BbCDR1 的缺失还导致在氧化、渗透、分生孢子壁破坏和紫外线胁迫下的分生孢子发芽率降低。有趣的是,与野生型相比,ΔBbCDR1 表现出真菌毒力增加,而 LT50 降低 12.5%。结论进一步分析显示,BbCDR1 抑制 BbbrlA 的表达并激活 BbwetA,这是中枢发育途径 (CDP) 的两个组成部分,分别调节分生孢子起始和成熟。这些发现表明 BbCDR1 在分生孢子发育中起着至关重要的作用,并且是高活性真菌杀虫剂基因工程的新靶基因。© 2025 化工学会.
更新日期:2025-01-24
中文翻译:
![](https://scdn.x-mol.com/jcss/images/paperTranslation.png)
转录因子 BbCDR1 调节昆虫病原真菌 Beauveria bassiana 分生孢子形成和成熟之间的协调
背景昆虫病原真菌 Beauveria bassiana 已被广泛用于害虫生物防治,其中分生孢子是主要的活性剂。分生孢子产量和质量是真菌分生孢子发育中的两个重要特征,然而,协调分生孢子形成和发育的调节机制尚不清楚。结果在本研究中,我们鉴定了一种 Zn2Cys6 转录因子 BbCDR1,该转录因子在促进分生孢子成熟的同时抑制分生孢子的产生。与野生型相比,ΔBbCDR1 突变体的分生孢子产量增加了 1.88 倍,但分生孢子质量降低,包括细胞壁完整性和海藻糖合成降低。BbCDR1 的缺失还导致在氧化、渗透、分生孢子壁破坏和紫外线胁迫下的分生孢子发芽率降低。有趣的是,与野生型相比,ΔBbCDR1 表现出真菌毒力增加,而 LT50 降低 12.5%。结论进一步分析显示,BbCDR1 抑制 BbbrlA 的表达并激活 BbwetA,这是中枢发育途径 (CDP) 的两个组成部分,分别调节分生孢子起始和成熟。这些发现表明 BbCDR1 在分生孢子发育中起着至关重要的作用,并且是高活性真菌杀虫剂基因工程的新靶基因。© 2025 化工学会.