当前位置: X-MOL 学术Water Res. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Unveiling significant regrowth and potential risk of nontuberculous mycobacteria in hospital water supply system
Water Research ( IF 11.4 ) Pub Date : 2025-01-22 , DOI: 10.1016/j.watres.2025.123188
Yalan Gan, Futoshi Kurisu, Dai Simazaki, Mitsunori Yoshida, Hanako Fukano, Takeshi Komine, Hiromi Nagashima, Yoshihiko Hoshino, Ikuro Kasuga

The health burden of waterborne nontuberculous mycobacteria (NTM) is a rising concern. While the water supply systems can serve as a potential reservoir for NTM, their abundance, diversity, and transmission pathways remain unknown. This study aimed to characterize the prevalence and regrowth of NTM in building water supply system in a hospital where many M. abscessus were isolated from patients. The depletion of residual chlorine after stagnation and supply of warm water at the point of use promoted significant microbial regrowth, including NTM, in the hospital. The absolute abundance of Mycobacterium spp. 16S rRNA genes in tap water and shower water samples increased to approximately 104 copies/mL, while it was below the quantification limit in the finished water from a drinking water treatment plant. Amplicon sequencing of NTM-specific hsp65 genes revealed that M. abscessus was prevalent in all samples, while the dominant NTM species varied depending on locations even in the same building. The presence of M. abscessus in water suggested the possibility of waterborne transmission in the hospital. M. abscessus was frequently isolated from tap water, shower water, and shower biofilms. These isolates demonstrated high clonality and were closely affiliated with the ABS-GL4 cluster of M. abscessus subsp. abscessus. Even though the automatic mixing equipment at the point of use was replaced with new one, the settlement and growth of NTM were reproducibly observed, suggesting mixing equipment as a hotspot for NTM proliferation. Additional interventions including water quality control are required as the hospital water supply system is a hot spot for NTM regrowth.

中文翻译:


揭示医院供水系统中非结核分枝杆菌的显著再生和潜在风险



水生非结核分枝杆菌 (NTM) 的健康负担是一个日益受到关注的问题。虽然供水系统可以作为 NTM 的潜在水库,但它们的丰度、多样性和传播途径仍然未知。本研究旨在描述从患者身上分离出许多脓肿分枝杆菌的医院建筑供水系统中 NTM 的患病率和再生情况。停滞后余氯的消耗和使用点的温水供应促进了医院中显着的微生物再生,包括 NTM。自来水和淋浴水样品中分枝杆菌属 16S rRNA 基因的绝对丰度增加到约 104 拷贝/mL,而在饮用水处理厂的成品水中则低于定量限。NTM 特异性 hsp65 基因的扩增子测序显示,脓肿分枝杆菌在所有样本中都普遍存在,而优势的 NTM 物种因位置而异,即使在同一建筑物中也是如此。水中存在脓肿分枝杆菌表明医院可能存在水源传播。M. abscessus 经常从自来水、淋浴水和淋浴生物膜中分离出来。这些分离株表现出高克隆性,并且与脓肿分枝杆菌亚种的 ABS-GL4 簇密切相关。尽管使用点的自动混合设备被更换为新的自动混合设备,但可重复地观察到 NTM 的沉降和生长,这表明混合设备是 NTM 增殖的热点。由于医院供水系统是 NTM 再生的热点,因此需要额外的干预措施,包括水质控制。
更新日期:2025-01-22
down
wechat
bug