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Metagenomic and enzymatic mechanisms underpinning efficient water treatment of 2-ethylhexyl diphenyl phosphate (EHDPP) by the microbial consortium 8-ZY
Water Research ( IF 11.4 ) Pub Date : 2025-01-21 , DOI: 10.1016/j.watres.2025.123178
Yuanyuan Yu, Wantang Huang, Shaoyu Tang, Ying Xiang, Lizhu Yuan, Xifen Zhu, Hua Yin, Zhi Dang, Junfeng Niu

The ubiquitous presence, potential toxicity, and persistence of 2-ethylhexyl diphenyl phosphate (EHDPP) in the environment have raised significant concerns. In this study, we successfully isolate a novel microbial consortium, named 8-ZY, and we demonstrate its remarkable ability to degrade EHDPP using an extremely low concentration of the inoculate. A total of 11 degradation metabolites were identified, including hydrolysis, hydroxylated, methylated, glucuronide-conjugated, and previously unreported byproducts, enabling us to propose new transformation pathways. Further, we unveiled the active members of the microbial consortium 8-ZY during the degradation of EHDPP. We observed the presence of diverse active populations, which included Bradyrhizobium, Rhodopseudomonas, Sphingomonas, Hyphomicrobium, Chitinophaga, Aminobacter, and Ralstonia. A metagenomic analysis revealed the presence of genes that encode phosphatase, phosphodiesterase, cytochrome P450, and hydroxylase enzymes, thus indicating their crucial role in EHDPP degradation. Furthermore, we successfully isolated Burkholderia cepacia ZY1, Sphingopyxis terrae ZY2, and Amycolatopsis ZY3 from the 8-ZY consortium, confirming their significance in EHDPP degradation and metabolite formation. These findings underscored the diversity of strains and functional genes responsible for the transformation of EHDPP within the consortium 8-ZY, highlighting the essential role of synergistic interactions during EHDPP biodegradation processes. Molecular docking and dynamics simulation suggested that alkaline phosphatase, cytochrome P450, and hydroxylase stably bonded to EHDPP within their respective active pockets, targeting distinct sites on the EHDPP molecule. These findings provide a comprehensive understanding of the transformation mechanisms of OPEs and contribute valuable insights into their fate in the environment.

中文翻译:


微生物联盟 8-ZY 对 2-乙基己基二苯基磷酸酯 (EHDPP) 进行高效水处理的宏基因组和酶机制



2-乙基己基磷酸二苯酯 (EHDPP) 在环境中的普遍存在、潜在毒性和持久性引起了人们的严重关注。在这项研究中,我们成功分离了一种名为 8-ZY 的新型微生物联盟,并证明了其使用极低浓度的接种物降解 EHDPP 的显着能力。共鉴定出 11 种降解代谢物,包括水解、羟基化、甲基化、葡萄糖醛酸结合和以前未报道的副产物,使我们能够提出新的转化途径。此外,我们揭示了 EHDPP 降解过程中微生物联盟 8-ZY 的活性成员。我们观察到不同活跃种群的存在,包括 Bradyrhizobium、Rhodopseudomonas、Sphingomonas、Hyphomicrobium、Chitinophaga、Aminobacter 和 Ralstonia。宏基因组分析揭示了编码磷酸酶、磷酸二酯酶、细胞色素 P450 和羟化酶的基因的存在,从而表明它们在 EHDPP 降解中起着关键作用。此外,我们成功地从 8-ZY 联盟中分离出洋葱伯克霍尔德菌 ZY1 、 鞘状鞘呤 ZY2 和支链淀粉 ZY3 ,证实了它们在 EHDPP 降解和代谢物形成中的重要性。这些发现强调了 8-ZY 联盟中负责 EHDPP 转化的菌株和功能基因的多样性,突出了 EHDPP 生物降解过程中协同相互作用的重要作用。分子对接和动力学模拟表明,碱性磷酸酶、细胞色素 P450 和羟化酶在其各自的活性口袋内与 EHDPP 稳定结合,靶向 EHDPP 分子上的不同位点。 这些发现提供了对 OPE 转化机制的全面理解,并为它们在环境中的命运提供了有价值的见解。
更新日期:2025-01-21
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