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Combined application of methyl jasmonate and Bacillus subtilisLLCG89 suppresses blue and green mold of lemon (Citrus limon L.)
Pest Management Science ( IF 3.8 ) Pub Date : 2025-01-17 , DOI: 10.1002/ps.8643
Anam Moosa, Faisal Zulfiqar, Mohammed O. Alshaharni, Khairiah Mubarak Alwutayd, Layla A Alahmari, Seham Sater Alhelaify, Ohud Muslat Alharthy, Eman Fayad, Fahad Al‐Asmari, Alaa Baazeem, Rasha M. Alzayed, Sondos A. Alhajouj, Tahir Mahmood

BackgroundBacillus species produce antimicrobial lipopeptides (LPs) and methyl jasmonate (MeJA) induces resistance in harvested fruits against postharvest pathogens. However, there is limited evidence of the combined efficacy of Bacillus LPs and MeJA to suppress postharvest diseases.ResultsThis study presents the combined effect of Bacillus LPs and MeJA to suppress P. digitatum and P. italicum associated with green and blue mold of lemon. Eight Bacillus strains were screened in a direct antagonism plate assay, where Bacillus subtilis LLCG89 and B. atrophaeus HFZ23 exhibited the highest inhibition of both molds. Subsequently, in an in vitro assay, LPs extracted from LLCG89 demonstrated the highest antifungal activity against both pathogens compared to HFZ23. When combined with MeJA, LLCG89 LPs reduced the lesion diameter and disease incidence of blue and green mold on lemon fruit. Co‐application of MeJA and LLCG89 LPs decreased the levels of MDA, H2O2, and electrolyte leakage compared to single treatments and controls. Furthermore, treatment with MeJA and LLCG89 LPs increased the activities of defense enzymes including SOD, CAT, POD, PPO, CHI, GLU, APX, and PAL with peak activity between 48 to 72 h. The co‐application of MeJA and LLCG89 LPs showed the highest enzyme activity compared to the control. Postharvest quality analysis indicated that co‐application of LLCG89 LPs and MeJA showed little impact on fruit quality.ConclusionThe findings of our study highlight the potential of Bacillus‐derived LPs and MeJA as effective tools for managing the Penicillium decay of lemons by maintaining fruit quality. © 2025 Society of Chemical Industry.

中文翻译:


茉莉酸甲酯和枯草芽孢杆菌 LLCG89 的联合应用可抑制柠檬 (Citrus limon L.) 的蓝色和绿色霉菌



背景芽孢杆菌物种产生抗菌脂肽 (LP),茉莉酸甲酯 (MeJA) 诱导收获的水果对收获后病原体的抗性。然而,关于芽孢杆菌 LPs 和 MeJA 对抑制采后疾病的综合疗效的证据有限。结果本研究介绍了芽孢杆菌 LPs 和 MeJA 对抑制与柠檬绿蓝霉菌相关的趾状疟原虫和意大利假单胞菌的综合作用。在直接拮抗板测定中筛选了 8 株芽孢杆菌菌株,其中枯草芽孢杆菌 LLCG89 和萎缩芽孢杆菌 HFZ23 对两种霉菌的抑制作用最高。随后,在体外测定中,与 HFZ23 相比,从 LLCG89 中提取的 LP 对两种病原体表现出最高的抗真菌活性。当与 MeJA 联合使用时,LLCG89 LPs 减少了柠檬果实上病变直径和蓝色和绿色霉菌的病害发生率。与单一治疗和对照相比,MeJA 和 LLCG89 LPs 的共同应用降低了 MDA 、 H 2 O 2 和电解质渗漏的水平。此外,用 MeJA 和 LLCG89 LPs 处理增加了防御酶的活性,包括 SOD、CAT、POD、PPO、CHI、GLU、APX 和 PAL,峰值活性在 48 至 72 小时之间。与对照相比,MeJA 和 LLCG89 LPs 的共同应用显示出最高的酶活性。采后品质分析表明,LLCG89 LPs 和 MeJA 的共同施用对果实品质影响不大。结论我们的研究结果强调了芽孢杆菌衍生的 LPs 和 MeJA 作为通过保持水果品质来管理柠檬青霉腐烂的有效工具的潜力。© 2025 化工学会.
更新日期:2025-01-17
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