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Decipher syntrophies and adaptive response towards enhancing conversion of propionate to methane under psychrophilic condition
Water Research ( IF 11.4 ) Pub Date : 2025-01-16 , DOI: 10.1016/j.watres.2025.123143
Miao Yan, Zhijian Shi, Xinjie Zhang, Xiaofeng Lin, Yongming Sun, Xingyu Cheng, Hailin Tian, Ying Li

Propionate is a key intermediate in anaerobic digestion (AD) under low operational temperatures, which can destabilize the process. In this study, the supplementation of syntrophic cold-tolerant consortia and trace elements significantly improved the performance of psychrophilic (20 °C) reactor, increasing methane production to 91 % of mesophilic reactor levels and reducing propionate concentrations to less than 2 % of those in untreated psychrophilic reactors. Multi-omics analyses revealed that psychrophilic conditions downregulated the methylmalonyl-CoA and aceticlastic methanogenesis pathways. Electron paramagnetic resonance analyses detected 2.6E-05mol/L reactive oxygen species as stress metabolites in the inhibited psychrophilic reactors. Conversely, supplementation with syntrophic cold-tolerant consortia and trace elements enhanced the abundance of Smithellaceae, Syntrophobacteraceae, and Methanothrix by fivefold in the bioenhanced reactors. This supplementation broadened the propionate degradation pathways from relying solely on the methylmalonyl-CoA pathway to also incorporating the dismutation pathway, while upregulating both pathways. These changes enhanced methanogenesis from propionate through improved activity of the syntrophic cold-tolerant consortia. Genome-centric metatranscriptomic analysis identified the upregulation of key antioxidant genes (sod, kat, grx), temperature regulation genes (cspA), and cryoprotective genes (pslF, pslH, cysE) within the syntrophic cold-tolerant consortia. Additionally, extracellular polymeric substance (EPS) yield per cell increased in the bioenhanced reactors by up to 1.07-fold compared to RC-P. These metabolic traits emphasize the critical roles in mitigating oxidative stress, adapting to low temperatures, and supporting efficient methanogenesis under psychrophilic conditions. These findings offer insights into the transcriptional responses and adaptive mechanisms of propionate-degrading consortia in response to psychrophilic stress.

中文翻译:


破译嗜冷条件下增强丙酸盐向甲烷转化的共养和适应性反应



丙酸盐是低温下厌氧消化 (AD) 的关键中间体,会破坏工艺的稳定性。在这项研究中,补充共养耐寒财团和微量元素显着改善了嗜冷 (20 °C) 反应器的性能,将甲烷产量提高到嗜温反应器水平的 91%,并将丙酸盐浓度降低到未经处理的嗜冷反应器水平的 2% 以下。多组学分析显示,嗜冷条件下调甲基丙二酰辅酶 A 和乙裂石性甲烷生成途径。电子顺磁共振分析在受抑制的嗜冷反应器中检测到 2.6E-05mol/L 活性氧作为应激代谢物。相反,补充共养耐寒财团和微量元素使生物增强反应器中 Smithellaceae、Syntrophobacteraceae 和 Methanothrix 的丰度提高了五倍。这种补充剂拓宽了丙酸降解途径,从仅依赖于甲基丙二酰辅酶 A 途径扩展到也纳入了歧化途径,同时上调了这两种途径。这些变化通过提高共养耐寒财团的活性来增强丙酸盐的甲烷生成。以基因组为中心的元转录组学分析确定了共养耐寒联盟中关键抗氧化基因 (sod、kat、grx)、温度调节基因 (cspA) 和冷冻保护基因 (pslF、pslH、cysE) 的上调。此外,与 RC-P 相比,生物增强反应器中每个细胞的细胞外聚合物物质 (EPS) 产量提高了 1.07 倍。 这些代谢特性强调了在减轻氧化应激、适应低温和支持嗜冷条件下有效甲烷生成的关键作用。这些发现为丙酸盐降解财团响应嗜冷应激的转录反应和适应机制提供了见解。
更新日期:2025-01-16
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