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The cross‐resistance to etofenprox in Nilaparvata lugens with a high adaptability to resistant rice variety IR56
Pest Management Science ( IF 3.8 ) Pub Date : 2025-01-16 , DOI: 10.1002/ps.8668
Xiaowei Yuan, Tianshun He, Jianzheng Zou, Huihui Zhang, Zewen Liu
Pest Management Science ( IF 3.8 ) Pub Date : 2025-01-16 , DOI: 10.1002/ps.8668
Xiaowei Yuan, Tianshun He, Jianzheng Zou, Huihui Zhang, Zewen Liu
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BACKGROUNDThe application of resistant rice varieties and insecticides represents two crucial strategies for managing the brown planthopper (BPH), Nilaparvata lugens (Stål). Insects often employ similar detoxification mechanisms to metabolize plant secondary metabolites and insecticides, which poses a potential risk that BPH population adapted to resistant rice may also obtain resistance to some insecticides.RESULTSHere in a BPH population (R‐IR56) that has adapted to the resistant rice variety IR56 through continuous selection, the moderate resistance to etofenprox was observed. Insect P450s often contribute to insecticide resistance in insects. Through transcriptome sequencing of R‐IR56 and control populations, it was found that two P450 genes, CYP439A1 and CYP439A2 , were over‐expressed in R‐IR56. RNA interference confirmed the importance of two P450s in etofenprox resistance in vivo . The metabolite identification and catalytic activity of recombinant P450 proteins revealed the metabolism of etofenprox by both CYP439A1 and CYP439A2 in vitro , which catalyzed an O‐de‐ethylation on etofenprox. However, the up‐regulation of two P450 genes did not contribute to the adaptation of BPH to IR56 variety.CONCLUSIONThe study reveals that BPH adapted to resistant rice variety IR56 exhibits a moderate level of resistance to etofenprox, but the cross‐resistance to etofenprox in BPH is unidirectional. The findings here provide theoretical guidance for the integrated pest management strategy, especially focusing on the interplay between applications of resistant variety and insecticides. © 2025 Society of Chemical Industry.
中文翻译:
对抗性水稻品种 IR56 具有高度适应性的 Nilaparvata lugens 对 etofenprox 的交叉抗性
背景抗性水稻品种和杀虫剂的应用代表了管理褐飞虱 (BPH) Nilaparvata lugens (Stål) 的两种关键策略。昆虫通常采用类似的解毒机制来代谢植物次生代谢产物和杀虫剂,这构成了适应抗性水稻的 BPH 种群也可能对某些杀虫剂产生抗性的潜在风险。结果在通过连续选择适应抗性水稻品种 IR56 的 BPH 种群 (R-IR56) 中,观察到对依托芬普罗克斯的中度抗性。昆虫 P450 通常会导致昆虫对杀虫剂产生耐药性。通过 R-IR56 和对照群体的转录组测序,发现 CYP439A1 和 CYP439A2 两个 P450 基因在 R-IR56 中过表达。RNA 干扰证实了两个 P450 在体内 etofenprox 耐药性中的重要性。重组 P450 蛋白的代谢物鉴定和催化活性揭示了 CYP439A1 和 CYP439A2 在体外代谢 etofenprox,从而催化 etofenprox 上的 O-去乙基化。然而,两个 P450 基因的上调无助于 BPH 对 IR56 品种的适应。结论研究表明,适应抗性水稻品种 IR56 的 BPH 对 etofenprox 表现出中等水平的抗性,但 BPH 对 etofenprox 的交叉耐药性是单向的。这里的研究结果为病虫害综合治理策略提供了理论指导,特别是关注抗性品种和杀虫剂应用之间的相互作用。© 2025 化工学会.
更新日期:2025-01-16
中文翻译:
![](https://scdn.x-mol.com/jcss/images/paperTranslation.png)
对抗性水稻品种 IR56 具有高度适应性的 Nilaparvata lugens 对 etofenprox 的交叉抗性
背景抗性水稻品种和杀虫剂的应用代表了管理褐飞虱 (BPH) Nilaparvata lugens (Stål) 的两种关键策略。昆虫通常采用类似的解毒机制来代谢植物次生代谢产物和杀虫剂,这构成了适应抗性水稻的 BPH 种群也可能对某些杀虫剂产生抗性的潜在风险。结果在通过连续选择适应抗性水稻品种 IR56 的 BPH 种群 (R-IR56) 中,观察到对依托芬普罗克斯的中度抗性。昆虫 P450 通常会导致昆虫对杀虫剂产生耐药性。通过 R-IR56 和对照群体的转录组测序,发现 CYP439A1 和 CYP439A2 两个 P450 基因在 R-IR56 中过表达。RNA 干扰证实了两个 P450 在体内 etofenprox 耐药性中的重要性。重组 P450 蛋白的代谢物鉴定和催化活性揭示了 CYP439A1 和 CYP439A2 在体外代谢 etofenprox,从而催化 etofenprox 上的 O-去乙基化。然而,两个 P450 基因的上调无助于 BPH 对 IR56 品种的适应。结论研究表明,适应抗性水稻品种 IR56 的 BPH 对 etofenprox 表现出中等水平的抗性,但 BPH 对 etofenprox 的交叉耐药性是单向的。这里的研究结果为病虫害综合治理策略提供了理论指导,特别是关注抗性品种和杀虫剂应用之间的相互作用。© 2025 化工学会.