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Novel genomic features in entomopathogenic fungus Beauveria bassiana ILB308: accessory genomic regions and putative virulence genes involved in the infection process of soybean pest Piezodorus guildinii
Pest Management Science ( IF 3.8 ) Pub Date : 2025-01-11 , DOI: 10.1002/ps.8631
Héctor Oberti, Lucia Sessa, Carolina Oliveira‐Rizzo, Andrés Di Paolo, Andrea Sanchez‐Vallet, Michael F. Seidl, Eduardo Abreo
Pest Management Science ( IF 3.8 ) Pub Date : 2025-01-11 , DOI: 10.1002/ps.8631
Héctor Oberti, Lucia Sessa, Carolina Oliveira‐Rizzo, Andrés Di Paolo, Andrea Sanchez‐Vallet, Michael F. Seidl, Eduardo Abreo
BACKGROUNDBiological control methods involving entomopathogenic fungi like Beauveria bassiana have been shown to be a valuable approach in integrated pest management as an environmentally friendly alternative to control pests and pathogens. Identifying genetic determinants of pathogenicity in B. bassiana is instrumental for enhancing its virulence against insects like the resistant soybean pest Piezodorus guildinii . This study focused on comparative genomics of different B. bassiana strains and gene expression analyses to identify virulence genes in the hypervirulent strain ILB308, especially in response to infection of P. guildinii and growth on hydrocarbon HC15, a known virulence enhancer.RESULTSStrain ILB308 showed the highest number of virulence‐related features, such as candidate virulence proteins, effectors, small secreted proteins and biosynthetic gene clusters. ILB308 also had a high percentage of unique DNA sequences, including six accessory scaffolds. Gene expression analysis at 4 days post inoculation revealed upregulation of known virulence factors, including Tudor domain proteins, LysM motif‐containing proteins, subtilisin‐like proteases and novel genes encoding secreted effectors and heat‐labile enterotoxins. Growth on HC15 led to the upregulation of genes associated with oxidoreductase activity related to cuticular alkane degradation and fermentation metabolism/antioxidant responses in the hemolymph. The low number of known B. bassiana virulence genes points to novel or unknown mechanisms acting on the interaction between P. guildinii and strain ILB308.CONCLUSIONThe presence of accessory genomic regions and unique virulence genes in ILB308 may contribute to its higher virulence. These genes could be considered as potential targets for enhancing fungal virulence through genetic manipulation. © 2025 Society of Chemical Industry.
中文翻译:
昆虫病原真菌 Beauveria bassiana ILB308 的新基因组特征:参与大豆害虫 Piezodorus guildinii 感染过程的辅助基因组区域和推定毒力基因
背景涉及虫病原真菌(如球孢白僵菌)的生物学防治方法已被证明是综合害虫管理的一种有价值的方法,是控制害虫和病原体的环保替代方案。鉴定 B. bassiana 致病性的遗传决定因素有助于增强其对抗性大豆害虫 Piezodorus guildinii 等昆虫的毒力。本研究侧重于不同 B. bassiana 菌株的比较基因组学和基因表达分析,以鉴定高毒力菌株 ILB308 中的毒力基因,特别是响应 P. guildinii 的感染和碳氢化合物 HC15 上的生长,一种已知的毒力增强剂。结果菌株 ILB308 显示出最多的毒力相关特征,例如候选毒力蛋白、效应子、小分泌蛋白和生物合成基因簇。ILB308 还具有高比例的独特 DNA 序列,包括 6 个辅助支架。接种后 4 天的基因表达分析显示已知毒力因子上调,包括 Tudor 结构域蛋白、含有 LysM 基序的蛋白质、枯草杆菌蛋白酶样蛋白酶和编码分泌效应子和热不稳定肠毒素的新基因。HC15 上的生长导致与血淋巴中表皮烷烃降解和发酵代谢/抗氧化反应相关的氧化还原酶活性相关基因的上调。已知 B. bassiana 毒力基因的数量较少,这表明作用于 P. guildinii 和菌株 ILB308 之间相互作用的新型或未知机制。结论ILB308 中存在辅助基因组区域和独特的毒力基因可能是其较高毒力的原因。 这些基因可以被认为是通过基因操作增强真菌毒力的潜在靶标。© 2025 化工学会.
更新日期:2025-01-11
中文翻译:
昆虫病原真菌 Beauveria bassiana ILB308 的新基因组特征:参与大豆害虫 Piezodorus guildinii 感染过程的辅助基因组区域和推定毒力基因
背景涉及虫病原真菌(如球孢白僵菌)的生物学防治方法已被证明是综合害虫管理的一种有价值的方法,是控制害虫和病原体的环保替代方案。鉴定 B. bassiana 致病性的遗传决定因素有助于增强其对抗性大豆害虫 Piezodorus guildinii 等昆虫的毒力。本研究侧重于不同 B. bassiana 菌株的比较基因组学和基因表达分析,以鉴定高毒力菌株 ILB308 中的毒力基因,特别是响应 P. guildinii 的感染和碳氢化合物 HC15 上的生长,一种已知的毒力增强剂。结果菌株 ILB308 显示出最多的毒力相关特征,例如候选毒力蛋白、效应子、小分泌蛋白和生物合成基因簇。ILB308 还具有高比例的独特 DNA 序列,包括 6 个辅助支架。接种后 4 天的基因表达分析显示已知毒力因子上调,包括 Tudor 结构域蛋白、含有 LysM 基序的蛋白质、枯草杆菌蛋白酶样蛋白酶和编码分泌效应子和热不稳定肠毒素的新基因。HC15 上的生长导致与血淋巴中表皮烷烃降解和发酵代谢/抗氧化反应相关的氧化还原酶活性相关基因的上调。已知 B. bassiana 毒力基因的数量较少,这表明作用于 P. guildinii 和菌株 ILB308 之间相互作用的新型或未知机制。结论ILB308 中存在辅助基因组区域和独特的毒力基因可能是其较高毒力的原因。 这些基因可以被认为是通过基因操作增强真菌毒力的潜在靶标。© 2025 化工学会.