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Silencing of the plant‐derived horizontally transferred gene BtSC5DL effectively controls Bemisia tabaci MED
Pest Management Science ( IF 3.8 ) Pub Date : 2025-01-11 , DOI: 10.1002/ps.8638
Yifan Liu, Cheng Gong, Yuan Hu, Haolin Han, Tian Tian, Yili Luo, Xin Yang, Wen Xie, Qingjun Wu, Shaoli Wang, Zhaojiang Guo, Youjun Zhang

BACKGROUNDThe whitefly Bemisia tabaci is a notorious agricultural pest known for its ability to cause significant crop damage through direct feeding and virus transmission. Its remarkable adaptability and reproductive capacity are linked to its ability to acquire and integrate horizontally transferred genes (HTGs) into its genome. These HTGs increase the physiological and metabolic capacities of this pest, including cholesterol synthesis, which is critical for its survival and reproductive success. Among these genes, we identified a plant‐derived B. tabaci Δ7‐sterol C5‐desaturase‐like gene (BtSC5DL), which plays a pivotal role in B. tabaci cholesterol metabolism and reproductive biology.RESULTSIn this study, we cloned and identified the BtSC5DL gene from B. tabaci Mediterranean (MED). Bioinformatics and molecular analyses revealed that BtSC5DL was transferred from plants to B. tabaci millions of years ago and is now stably expressed in this species. Silencing BtSC5DL through dsRNA feeding resulted in significant reductions in egg production and cholesterol content in B. tabaci MED. Furthermore, virus‐induced gene silencing (VIGS) experiments confirmed that long‐term suppression of BtSC5DL had a notable ability to control whitefly populations.CONCLUSIONOur results demonstrate the crucial role of BtSC5DL in cholesterol biosynthesis in B. tabaci MED and suggest that the acquisition of this gene significantly enhances the reproductive capacity of this species. These findings provide a theoretical basis for the development of RNA interference (RNAi)‐based pest control strategies targeting BtSC5DL, offering a potential new approach for the effective management of whitefly populations in agricultural settings. © 2025 Society of Chemical Industry.

中文翻译:


沉默植物来源的水平转移基因 BtSC5DL 有效控制 Bemisia tabaci MED



背景粉虱 Bemisia tabaci 是一种臭名昭著的农业害虫,以其通过直接喂养和病毒传播造成严重作物损害的能力而闻名。其非凡的适应性和繁殖能力与其获取水平转移基因 (HTG) 并将其整合到基因组中的能力有关。这些 HTG 增加了这种害虫的生理和代谢能力,包括胆固醇合成,这对其生存和繁殖成功至关重要。在这些基因中,我们鉴定了一个植物来源的烟粉虱 Δ7-甾醇 C5-去饱和酶样基因 (BtSC5DL),它在烟粉虱碳水化合物代谢和生殖生物学中起关键作用。结果在本研究中,我们克隆并鉴定了地中海烟粉虱 (MED) 的 BtSC5DL 基因。生物信息学和分子分析表明,BtSC5DL 在数百万年前从植物转移到烟粉虱中,现已在该物种中稳定表达。通过 dsRNA 喂养沉默 BtSC5DL 导致 B. tabaci MED 中的产蛋量和胆固醇含量显着降低。此外,病毒诱导的基因沉默 (VIGS) 实验证实,长期抑制 BtSC5DL 具有显着的控制粉虱种群的能力。结论研究结果表明 BtSC5DL 在烟粉虱 MED 胆固醇合成中起关键作用,并表明该基因的获得显着增强了该物种的繁殖能力。这些发现为开发针对 BtSC5DL 的基于 RNA 干扰 (RNAi) 的害虫防治策略提供了理论基础,为农业环境中有效管理粉虱种群提供了一种潜在的新方法。© 2025 化工学会.
更新日期:2025-01-11
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