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A combination of genome mining with OSMAC strategy facilitates the discovery of bioactive metabolites produced from termite‐associated Streptomyces tanashiensisBYF‐112
Pest Management Science ( IF 3.8 ) Pub Date : 2025-01-11 , DOI: 10.1002/ps.8640
Jun Wu, YiHeng Wang, YaXuan Wang, XiaoHong Li, YueYue Li, Miao Zhang, JianHao Xiong, CaiPing Yin, ShuXiang Zhang, XinHua Liu, YingLao Zhang
Pest Management Science ( IF 3.8 ) Pub Date : 2025-01-11 , DOI: 10.1002/ps.8640
Jun Wu, YiHeng Wang, YaXuan Wang, XiaoHong Li, YueYue Li, Miao Zhang, JianHao Xiong, CaiPing Yin, ShuXiang Zhang, XinHua Liu, YingLao Zhang
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BACKGROUNDPreviously, eight new alkaloids were obtained from the fermentation extract of termite‐associated Streptomyces tanashiensis BYF‐112. However, genome analysis indicated the presence of many undiscovered secondary metabolites in S. tanashiensis BYF‐112.RESULTSHerein, 12 new alkaloids, tianwuine A–E (1–5), cephalandole C (6), venezuelines I–L (7–10), N ‐(4‐methylphenyl‐2‐hydroxy) formamide (11) and N ‐(5‐formyl‐2‐hydroxyphenyl) formamide (12), as well as three known metabolites (13–15) were discovered from BYF‐112 based on a combination of genome mining and the one strain many compounds (OSMAC) strategy. Plausible biosynthetic pathways of 1–13 were proposed using bioinformatic analysis of the full genome of BYF‐112. Partial metabolites were evaluated in vitro for their antibacterial, phytotoxic, and anti‐inflammatory activities. Pyrroloformamide A (14) showed strong antibacterial activities against Staphylococcus aureus , methicillin‐resistant Staphylococcus aureus , Pseudomonas syringae pv. actinidae , Xanthomonas oryzae pv. oryzae and Xanthomonas oryzae pv. oryzicola at a concentration of 50 μg per 6 mm disk. Simultaneously, pyrroloformamide A (14) also had a strong inhibitory effect on the radicle growth of Echinochloa crusgalli with an inhibition rate of 98.01% at a concentration of 100 μg/mL, equivalent to the positive 2,4‐dichlorophenoxyacetic acid. Subsequently, the possible herbicidal mechanism of 14 was explored using molecular docking simulation. In addition, venezueline G (13) displayed a strong inhibitory effect of NO production, with an half‐maximal inhibitory concentration (IC50 ) value of 2.3 μm , which was comparable with that of BAY 11‐7082 with an IC50 value of 2.1 μm .CONCLUSIONThese findings revealed a perspective for the development of novel bioactive drugs in the food, agricultural, and biomedical fields utilizing the metabolites of BYF‐112. © 2025 Society of Chemical Industry.
中文翻译:
基因组挖掘与 OSMAC 策略的结合有助于发现由白蚁相关的 Streptomyces tanashiensisBYF-112 产生的生物活性代谢物
背景从白蚁相关链霉菌 BYF-112 的发酵提取物中获得 8 种新的生物碱。然而,基因组分析表明,S. tanashiensis BYF-112 中存在许多未被发现的次生代谢物.结果基于基因组挖掘和一种菌株多化合物 (OSMAC) 的组合,从 BYF-112 中发现了 12 种新生物碱、天乌碱 A-E (1-5)、头孢菌 C (6)、委内瑞拉碱 I-L (7-10)、N-(4-甲基苯基-2-羟基)甲酰胺 (11) 和 N-(5-甲酰基-2-羟基苯基)甲酰胺 (12),以及三种已知的代谢物 (13-15) 是基于基因组挖掘和一种菌株多化合物 (OSMAC) 的组合从 BYF-112 中发现的策略。使用对 BYF-112 全基因组的生物信息学分析,提出了 1-13 的合理生物合成途径。在体外评估部分代谢物的抗菌、植物毒性和抗炎活性。吡咯二甲酰胺 A (14) 对金黄色葡萄球菌、耐甲氧西林金黄色葡萄球菌、丁香假单胞菌 pv 显示出很强的抗菌活性。放线菌科,Xanthomonas oryzae pv.水稻和水稻黄单胞菌 PV。每 6 mm 圆盘浓度为 50 μg的谷水。同时,吡咯双酰胺 A (14) 对棘爪棘皮的胚根生长也有很强的抑制作用,在 100 μg/mL 浓度下抑制率为 98.01%,相当于阳性 2,4-二氯苯氧乙酸。随后,利用分子对接模拟探索了 14 可能的除草机理。此外,委内瑞兰 G (13) 显示出强烈的 NO 产生抑制作用,半数最大抑制浓度 (IC50) 值为 2.3 μm,与 BAY 11-7082 的 IC50 值为 2.1 μm 相当。结论这些研究结果揭示了利用 BYF-112 的代谢物在食品、农业和生物医学领域开发新型生物活性药物的前景。© 2025 化工学会.
更新日期:2025-01-11
中文翻译:
![](https://scdn.x-mol.com/jcss/images/paperTranslation.png)
基因组挖掘与 OSMAC 策略的结合有助于发现由白蚁相关的 Streptomyces tanashiensisBYF-112 产生的生物活性代谢物
背景从白蚁相关链霉菌 BYF-112 的发酵提取物中获得 8 种新的生物碱。然而,基因组分析表明,S. tanashiensis BYF-112 中存在许多未被发现的次生代谢物.结果基于基因组挖掘和一种菌株多化合物 (OSMAC) 的组合,从 BYF-112 中发现了 12 种新生物碱、天乌碱 A-E (1-5)、头孢菌 C (6)、委内瑞拉碱 I-L (7-10)、N-(4-甲基苯基-2-羟基)甲酰胺 (11) 和 N-(5-甲酰基-2-羟基苯基)甲酰胺 (12),以及三种已知的代谢物 (13-15) 是基于基因组挖掘和一种菌株多化合物 (OSMAC) 的组合从 BYF-112 中发现的策略。使用对 BYF-112 全基因组的生物信息学分析,提出了 1-13 的合理生物合成途径。在体外评估部分代谢物的抗菌、植物毒性和抗炎活性。吡咯二甲酰胺 A (14) 对金黄色葡萄球菌、耐甲氧西林金黄色葡萄球菌、丁香假单胞菌 pv 显示出很强的抗菌活性。放线菌科,Xanthomonas oryzae pv.水稻和水稻黄单胞菌 PV。每 6 mm 圆盘浓度为 50 μg的谷水。同时,吡咯双酰胺 A (14) 对棘爪棘皮的胚根生长也有很强的抑制作用,在 100 μg/mL 浓度下抑制率为 98.01%,相当于阳性 2,4-二氯苯氧乙酸。随后,利用分子对接模拟探索了 14 可能的除草机理。此外,委内瑞兰 G (13) 显示出强烈的 NO 产生抑制作用,半数最大抑制浓度 (IC50) 值为 2.3 μm,与 BAY 11-7082 的 IC50 值为 2.1 μm 相当。结论这些研究结果揭示了利用 BYF-112 的代谢物在食品、农业和生物医学领域开发新型生物活性药物的前景。© 2025 化工学会.