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Investigating non‐target site resistance to pyroxsulam in a glyphosate‐resistant Lolium rigidum population
Pest Management Science ( IF 3.8 ) Pub Date : 2025-01-11 , DOI: 10.1002/ps.8636
Yalin Zeng, Min Liu, Hong Qian, Hui Zhao, Yuhang Fang, Qin Yu, Lianyang Bai, Lang Pan

BACKGROUNDResistance to multiple herbicides is common in Lolium rigidum. Here, resistance to acetolactate synthase (ALS)‐ and susceptibility to acetyl‐CoA carboxylase (ACCase)‐inhibiting herbicides was confirmed in a glyphosate‐resistant L. rigidum population (NLR70) from Australia and the mechanisms of pyroxsulam resistance were examined.RESULTSNo ALS target‐site mutations nor gene overexpression were detected. Cytochrome P450 monooxygenase (P450) and glutathione S‐transferase (GST) inhibitors (indicators of some certain P450s or GSTs) did not significantly affect the resistance to pyroxsulam. Nevertheless, HPLC analysis showed that plants of the NLR70 population metabolized pyroxsulam faster than plants of the herbicide‐susceptible population (SVLR1). RNA sequencing analysis and RT‐qPCR validation confirmed that four P450s (CYP709B2, CYP72A14, CYP89A2, CYP94B3), one GT (UGT79), and one ABC transporter (ABCG41) genes were constitutively upregulated in NLR70 plants.CONCLUSIONThis study demonstrates that the glyphosate‐resistant L. rigidum population (NLR70) also exhibits resistance to pyroxsulam and identifies six candidate genes associated with non‐target site resistance to pyroxsulam. © 2025 Society of Chemical Industry.

中文翻译:


研究耐草甘膦 Lolium rigidum 种群对吡咯磺草胺的非靶位点耐药性



背景对多种除草剂的耐受性在 Lolium rigidum 中很常见。在这里,在来自澳大利亚的草甘膦抗性 L. rigidum 种群 (NLR70) 中证实了对乙酰乳酸合酶 (ALS) 的耐药性和对乙酰辅酶 A 羧化酶 (ACCase) 抑制除草剂的敏感性,并检查了吡咯脲仑耐药的机制。结果未检测到 ALS 靶位点突变或基因过表达。细胞色素 P450 单加氧酶 (P450) 和谷胱甘肽 S-转移酶 (GST) 抑制剂 (一些某些 P450 或 GST 的指标) 对吡咯苏仑的耐药性没有显着影响。尽管如此,HPLC 分析表明,NLR70 种群的植物比除草剂敏感种群 (SVLR1) 的植物代谢吡咯磺兰胺更快。RNA 测序分析和 RT-qPCR 验证证实,4 个 P450 (CYP709B2、CYP72A14、CYP89A2、CYP94B3)、1 个 GT (UGT79) 和 1 个 ABC 转运蛋白 (ABCG41) 基因在 NLR70 植物中组成性上调。结论该研究表明,抗草甘膦的 L. rigidum 群体 (NLR70) 也表现出对 pyroxsulam 的耐药性,并鉴定了 6 个与非靶位点对 pyroxsulam 耐药相关的候选基因。© 2025 化工学会.
更新日期:2025-01-11
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