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Genotypic diversity and biological characterization of Alphabaculovirus mabrassicae isolates from a wide host range
Pest Management Science ( IF 3.8 ) Pub Date : 2025-01-10 , DOI: 10.1002/ps.8620
Xiaoqin Yang, Chengfeng Lei, Xiaowei Peng, Yuanqin Min, Jia Hu, Xiulian Sun

BACKGROUNDBaculoviruses are ideal biological insecticides, providing long‐lasting pest control and environmental benefits. Alphabaculovirus mabrassicae stains, with their broad host range, have been effective in agricultural pest management. Various A. mabrassicae isolates (MbMNPV‐CHb1/CTa/K1/QD, MyseMNPV‐Hb, HearMNPV and MacoNPV‐B) have been identified in different hosts. Identifying more effective A. mabrassicae strains with detailed genetic information is crucial for commercial use.RESULTSLaboratory bioassays showed that the median lethal concentration (LC50) of MyseMNPV‐Hb against Mythimna separata was significantly lower than those against Helicoverpa armigera and Spodoptera exigua, but higher than the LC50 of MbMNPV‐CHb1, MbMNPV‐QD and HearMNPV against H. armigera or S. exigua. Comparative genomic analysis revealed significant differences in genomic composition and single‐nucleotide polymorphisms between MyseMNPV‐Hb and the other isolates. A piggyBac‐like element, likely to have been from Alcis repandata (Lepidoptera: Geometridae), was identified in the genomes of these isolates. Eight genes in the A. mabrassicae genomes were found to be under positive selection.CONCLUSIONAlphabaculovirus mabrassicae isolates exhibit different infectivity in various pests, indicating the need for selecting appropriate isolates specific target pests. This study elucidates the genetic factors contributing to the differential infectivity of A. mabrassicae isolates and extends knowledge on its population characteristics. © 2025 Society of Chemical Industry.

中文翻译:


来自广泛宿主范围的 Alphabaculovirus mabrassicae 分离株的基因型多样性和生物学特征



背景针状病毒是理想的生物杀虫剂,可提供持久的害虫控制和环境效益。α 杆状病毒 mabrassicae 染色剂具有广泛的宿主范围,在农业害虫管理中一直很有效。已在不同的宿主中鉴定出各种 A. mabrassicae 分离株 (MbMNPV-CHb1/CTa/K1/QD、MyseMNPV-Hb、HearMNPV 和 MacoNPV-B)。通过详细的遗传信息确定更有效的 A. mabrassicae 菌株对于商业用途至关重要。结果生物测定结果MyseMNPV-Hb 对 Mythimna separata 的中位致死浓度 (LC50) 显著低于对 Helicoverpa armigera 和 Spodoptera exigua,但高于 MbMNPV-CHb1、MbMNPV-QD 和 HearMNPV 对 H. armigera 或 S. exiga 的 LC50。比较基因组分析显示 MyseMNPV-Hb 与其他分离株之间的基因组组成和单核苷酸多态性存在显著差异。在这些分离株的基因组中鉴定出一种类似 piggyBac 的元素,可能来自 Alcis repandata(鳞翅目:Geometridae)。发现 A. mabrassicae 基因组中的 8 个基因处于正向选择下。结论状病毒菌株在多种有害生物中表现出不同的传染性,需要选择合适的菌株来特异性目标害虫。本研究阐明了导致 A. mabrassicae 分离株传染性差异的遗传因素,并扩展了有关其种群特征的知识。© 2025 化工学会.
更新日期:2025-01-10
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