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Knockout of a testis‐specific gene cluster impairs male fertility in the fall armyworm, Spodoptera frugiperda
Pest Management Science ( IF 3.8 ) Pub Date : 2025-01-07 , DOI: 10.1002/ps.8634
Pei Wang, Yuchen Zhao, Yalan Cheng, Siyu Tian, Yuxuan Bai, Junfeng Zuo, Subba Reddy Palli, Xien Chen
Pest Management Science ( IF 3.8 ) Pub Date : 2025-01-07 , DOI: 10.1002/ps.8634
Pei Wang, Yuchen Zhao, Yalan Cheng, Siyu Tian, Yuxuan Bai, Junfeng Zuo, Subba Reddy Palli, Xien Chen
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BACKGROUNDThe function of some testis‐specific genes (TSGs) in model insects have been studied, but their function in non‐model insects remains largely unexplored. In the present study, we identified several TSGs in the fall armyworm (FAW), a significant agricultural pest, through comparative transcriptomic analysis. A testis‐specific gene cluster (TSGC) comprising multiple functional genes and long non‐coding RNAs was found.RESULTSQuantitative PCR analysis validated the testis‐specific expression of genes within this cluster across different developmental stages. Interspecies collinearity analysis indicated that this TSGC is conserved among lepidopterans. To elucidate the function of this TSGC, we employed the CRISPR/Cas9 gene‐editing technique to generate loss‐of‐function mutants. Notably, the hatching rate of eggs produced by wild‐type females mated with mutant males (approximately 30%) was substantially lower than that of eggs from wild‐type females mated with wild‐type males (approximately 80%). Additionally, a significant reduction in the size and number of sperm was observed in the testis of mutant males compared to wild‐type males.CONCLUSIONCollectively, this study not only elucidates the role of the TSGC in supporting male fertility in the FAW, which provides valuable information for understanding the mechanism of male reproduction in insects, but also identifies potential target genes for the development of genetic control strategies against lepidopteran pests. © 2025 Society of Chemical Industry.
中文翻译:
敲除睾丸特异性基因簇会损害草地贪夜蛾 (Spodoptera frugiperda) 的雄性生育能力
背景已经研究了模型昆虫中一些睾丸特异性基因 (TSG) 的功能,但它们在非模型昆虫中的功能在很大程度上仍未得到探索。在本研究中,我们通过比较转录组学分析在草地贪夜蛾 (FAW) 中鉴定了几种 TSG,这是一种重要的农业害虫。发现一个包含多个功能基因和长链非编码 RNA 的睾丸特异性基因簇 (TSGC)。结果定量 PCR 分析验证了该簇内基因在不同发育阶段的睾丸特异性表达。种间共线性分析表明,这种 TSGC 在鳞翅目中是保守的。为了阐明这种 TSGC 的功能,我们采用了 CRISPR/Cas9 基因编辑技术来产生功能丧失突变体。值得注意的是,野生型雌性与突变雄配产生的卵的孵化率 (约 30%) 大大低于野生型雌性与野生型雄配的卵 (约 80%)。此外,与野生型雄性相比,在突变雄性睾丸中观察到精子的大小和数量显着减少。结论本研究不仅阐明了 TSGC 在支持 FAW 雄性生育能力中的作用,为理解昆虫雄性繁殖机制提供了有价值的信息,而且还确定了针对鳞翅目害虫的遗传控制策略开发的潜在靶基因。© 2025 化工学会.
更新日期:2025-01-07
中文翻译:
![](https://scdn.x-mol.com/jcss/images/paperTranslation.png)
敲除睾丸特异性基因簇会损害草地贪夜蛾 (Spodoptera frugiperda) 的雄性生育能力
背景已经研究了模型昆虫中一些睾丸特异性基因 (TSG) 的功能,但它们在非模型昆虫中的功能在很大程度上仍未得到探索。在本研究中,我们通过比较转录组学分析在草地贪夜蛾 (FAW) 中鉴定了几种 TSG,这是一种重要的农业害虫。发现一个包含多个功能基因和长链非编码 RNA 的睾丸特异性基因簇 (TSGC)。结果定量 PCR 分析验证了该簇内基因在不同发育阶段的睾丸特异性表达。种间共线性分析表明,这种 TSGC 在鳞翅目中是保守的。为了阐明这种 TSGC 的功能,我们采用了 CRISPR/Cas9 基因编辑技术来产生功能丧失突变体。值得注意的是,野生型雌性与突变雄配产生的卵的孵化率 (约 30%) 大大低于野生型雌性与野生型雄配的卵 (约 80%)。此外,与野生型雄性相比,在突变雄性睾丸中观察到精子的大小和数量显着减少。结论本研究不仅阐明了 TSGC 在支持 FAW 雄性生育能力中的作用,为理解昆虫雄性繁殖机制提供了有价值的信息,而且还确定了针对鳞翅目害虫的遗传控制策略开发的潜在靶基因。© 2025 化工学会.