The wheat (Triticum spp.) stem rust pathogen, Puccinia graminis f. sp. tritici Eriks. and E. Henn. (Pgt), has continued to be a devastating biotic stress in wheat production. Over previous decades, scientists have identified several resistance genes effective against Pgt. However, the ever‐evolving Pgt and low availability of durable resistance necessitates continuous identification and wise deployment of resistance genes. To elucidate the identity of our previously reported stem rust resistance in hard red winter wheat cultivar Gage, we used recombinant inbred lines (RILs) developed from the cross of Bill Brown × Gage and evaluated them for 3 years for response to six different stem rust pathogen races individually at the seedling stage in the greenhouse and a mixture of these races in the field. Using molecular markers, we determined the genomic regions that affect stem rust resistance in Gage, which identified two quantitative trait loci (QTLs) at the seedling stage and one major QTL at the adult stage, giving insight into why Gage has superior stem rust resistance. The seedling stem rust resistance was from SrTmp and likely from an Sr7 allele. QTLs conferring adult plant resistance in Gage were mainly from Sr2, but molecular analysis suggested additional minor‐effect QTLs were involved.

中文翻译：

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Bill Brown/Gage 冬小麦种群茎锈病抗性 QTL 定位


小麦 （Triticum spp.） 茎锈病原菌 Puccinia graminis f. sp. tritici Eriks.和 E. Henn.（Pgt） 一直是小麦生产中毁灭性的生物胁迫。在过去的几十年里，科学家们已经确定了几个对 Pgt 有效的耐药基因。然而，不断发展的 Pgt 和持久耐药性的低可用性需要持续识别和明智地部署耐药基因。为了阐明我们之前报道的硬红冬小麦品种 Gage 的茎锈病抗性的身份，我们使用了从 Bill Brown × Gage 杂交开发的重组自交系 （RIL），并在温室幼苗阶段分别评估了它们对六种不同的茎锈病病原体种族的反应 3 年以及这些种族在田间的混合物。利用分子标记，我们确定了影响 Gage 茎锈病抗性的基因组区域，在幼苗期鉴定了两个数量性状位点 （QTL），在成年期鉴定了一个主要 QTL，从而深入了解了为什么 Gage 具有优异的茎锈病抗性。幼苗茎锈病抗性来自 SrTmp，可能来自 Sr7 等位基因。在 Gage 中赋予成年植物抗性的 QTL 主要来自 Sr2，但分子分析表明涉及其他次要效应 QTL。