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Alanine to glycine substitution in the PyR2 confers sodium channel resistance to Type I pyrethroids
Pest Management Science ( IF 3.8 ) Pub Date : 2024-12-31 , DOI: 10.1002/ps.8625
Mengli Chen, Likui Wang, Xiangyi Zhou, Guoxing Chen, Zhanyi Xu, Ru Yan, Jiali Qian, Guonian Zhu, Shaoying Wu, Huiming Wu
Pest Management Science ( IF 3.8 ) Pub Date : 2024-12-31 , DOI: 10.1002/ps.8625
Mengli Chen, Likui Wang, Xiangyi Zhou, Guoxing Chen, Zhanyi Xu, Ru Yan, Jiali Qian, Guonian Zhu, Shaoying Wu, Huiming Wu
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BACKGROUNDAedes aegypti is a primary urban vector of dengue, yellow fever, Zika and chikungunya worldwide. Pyrethroid insecticides are the most effective insecticides for controlling Ae. aegypti . However, pyrethroid resistance has developed due to the long‐term overuse of the insecticides, and many knockdown resistance (kdr) mutations have been identified in the resistant populations. A1007G, an alanine to glycine substitution, was found in resistant Ae. aegypti from Vietnam and Malaysia, which has always co‐existed with F1534C and V1016G. However, the role of A1007G in pyrethroid resistance and the linkage of A1007G and F1534C or V1016G remain unknown.RESULTSIn this study, we examined the effects of mutations on the sodium channel gating properties and pyrethroid sensitivity in Xenopus oocytes. We found mutations A1007G, A1007G + F1534C and A1007G + V1016G + F1534C shifted the voltage dependence of activation in the depolarizing direction. Mutations A1007G + F1534C and A1007G + V1016G + F1534C shifted the voltage dependence of inactivation in the depolarizing direction. Both mutations A1007G and F1534C reduced the channel sensitivity to two Type I pyrethroids, permethrin and bifenthrin, and synergistic effects were observed between mutations A1007G and F1534C. However, none of the mutations, A1007G, F1534C and A1007G + F1534C affected the channel sensitivity to two Type II pyrethroids, deltamethrin and cypermethrin. Furthermore, triple mutations A1007G + V1016G + F1534C significantly reduced the channel sensitivity to both Type I and Type II pyrethroids.CONCLUSIONWe identified A1007G had a distinct effect on sodium channel sensitivity to Type I, but not to Type II pyrethroids, also A1007G exhibited synergistic effects with F1534C to Type I pyrethroids, which will provide a fundamental insight into the distinct molecular interactions between insect sodium channel and Type I or Type II pyrethroids. © 2024 Society of Chemical Industry.
中文翻译:
PyR2 中丙氨酸到甘氨酸的取代赋予了对 I 型拟除虫菊酯的钠通道耐药性
背景埃及伊蚊是全球登革热、黄热病、寨卡病毒和基孔肯雅热的主要城市传播媒介。拟除虫菊酯类杀虫剂是控制 Ae 最有效的杀虫剂。埃及。然而,由于长期过度使用杀虫剂,已经产生了拟除虫菊酯耐药性,并且在耐药种群中发现了许多敲除抗性 (kdr) 突变。A1007G 是一种丙氨酸到甘氨酸的替代物,在耐药性 Ae 中被发现。来自越南和马来西亚的埃及,它一直与 F1534C 和 V1016G 共存。然而,A1007G 在拟除虫菊酯耐药性中的作用以及 A1007G 和 F1534C 或 V1016G 的键仍然未知。结果在本研究中,我们检查了突变对非洲爪蟾卵母细胞钠通道门控特性和拟除虫菊酯敏感性的影响。我们发现突变 A1007G 、 A1007G + F1534C 和 A1007G + V1016G + F1534C 将激活的电压依赖性向去极化方向移动。突变 A1007G + F1534C 和 A1007G + V1016G + F1534C 将失活的电压依赖性向去极化方向移动。突变 A1007G 和 F1534C 都降低了对两种 I 型拟除虫菊酯(氯菊酯和联苯菊酯)的通道敏感性,并且在突变 A1007G 和 F1534C 之间观察到协同效应。然而,A1007G 、 F1534C 和 A1007G + F1534C 突变均未影响对两种 II 型拟除虫菊酯 δmethrin 和 cyperperrin 的通道敏感性。此外,三重突变 A1007G + V1016G + F1534C 显著降低了对 I 型和 II 型拟除虫菊酯的通道敏感性。结论我们发现 A1007G 对钠通道对 I 型拟除虫菊酯的敏感性有明显影响,但对 II 型拟除虫菊酯没有影响,A1007G 与 F1534C 对 I 型拟除虫菊酯表现出协同作用,这将为昆虫钠通道与 I 型或 II 型拟除虫菊酯之间独特的分子相互作用提供根本性的见解。© 2024 化工学会.
更新日期:2024-12-31
中文翻译:
![](https://scdn.x-mol.com/jcss/images/paperTranslation.png)
PyR2 中丙氨酸到甘氨酸的取代赋予了对 I 型拟除虫菊酯的钠通道耐药性
背景埃及伊蚊是全球登革热、黄热病、寨卡病毒和基孔肯雅热的主要城市传播媒介。拟除虫菊酯类杀虫剂是控制 Ae 最有效的杀虫剂。埃及。然而,由于长期过度使用杀虫剂,已经产生了拟除虫菊酯耐药性,并且在耐药种群中发现了许多敲除抗性 (kdr) 突变。A1007G 是一种丙氨酸到甘氨酸的替代物,在耐药性 Ae 中被发现。来自越南和马来西亚的埃及,它一直与 F1534C 和 V1016G 共存。然而,A1007G 在拟除虫菊酯耐药性中的作用以及 A1007G 和 F1534C 或 V1016G 的键仍然未知。结果在本研究中,我们检查了突变对非洲爪蟾卵母细胞钠通道门控特性和拟除虫菊酯敏感性的影响。我们发现突变 A1007G 、 A1007G + F1534C 和 A1007G + V1016G + F1534C 将激活的电压依赖性向去极化方向移动。突变 A1007G + F1534C 和 A1007G + V1016G + F1534C 将失活的电压依赖性向去极化方向移动。突变 A1007G 和 F1534C 都降低了对两种 I 型拟除虫菊酯(氯菊酯和联苯菊酯)的通道敏感性,并且在突变 A1007G 和 F1534C 之间观察到协同效应。然而,A1007G 、 F1534C 和 A1007G + F1534C 突变均未影响对两种 II 型拟除虫菊酯 δmethrin 和 cyperperrin 的通道敏感性。此外,三重突变 A1007G + V1016G + F1534C 显著降低了对 I 型和 II 型拟除虫菊酯的通道敏感性。结论我们发现 A1007G 对钠通道对 I 型拟除虫菊酯的敏感性有明显影响,但对 II 型拟除虫菊酯没有影响,A1007G 与 F1534C 对 I 型拟除虫菊酯表现出协同作用,这将为昆虫钠通道与 I 型或 II 型拟除虫菊酯之间独特的分子相互作用提供根本性的见解。© 2024 化工学会.