A novel Pickering interfacial biocatalysis (PIB) system has been, for the first time, successfully applied for the enzymatic selective hydrolysis of algae oils and fish oils to enrich n-3 PUFAs glycerides. Lipase AY 400SD was identified and adsorbed on hydrophobic hollow core-shell silica nanoparticles, resulting in the formation of the immobilized enzyme AY 400SD@HMSS-C₁₈. The biocatalyst was employed as an emulsifier to stabilize the water-in-oil Pickering emulsion, resulting in the successful construction of the PIB system. The newly developed PIB system resulted in a notable enhancement of the n-3 PUFAs content of the six oils, with a mean increase ranging from 9.17 % to 23.09 %, and with the recovery rate of n-3 PUFAs exceeded 90 %. The platform demonstrated to be stable and recyclable. The present research illustrated that the PIB has the potential to be a viable alternative for the purpose of enhancing the content of n-3 PUFAs in glycerides.

中文翻译：

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在 Pickering 乳液中通过界面生物催化高效酶促富集 n-3 多不饱和脂肪酸甘油酯


一种新的 Pickering 界面生物催化 （PIB） 系统首次成功应用于藻油和鱼油的酶促选择性水解，以富集 n-3 PUFA 甘油酯。脂肪酶 AY 400SD 被鉴定并吸附在疏水性空心壳二氧化硅纳米颗粒上，导致形成固定化酶 AY 400SD@HMSS-C₁₈。生物催化剂被用作乳化剂来稳定油包水 Pickering 乳液，从而成功构建了 PIB 系统。新开发的 PIB 系统使 6 种油的 n-3 PUFAs 含量显著提高，平均增加 9.17 % 至 23.09 %，n-3 PUFAs 的回收率超过 90 %。该平台被证明是稳定且可回收的。目前的研究表明，PIB 有可能成为提高甘油酯中 n-3 多不饱和脂肪酸含量的可行替代品。