Phosphatidylethanolamine (PE) is a ubiquitous lipid species in higher eukaryotes which resides preferentially in mitochondria. Here, we synthesized a multifunctionalized PE derivative (1) de-signed to identify PE-binding proteins in intact cells through photo-crosslinking and subsequent isolation and proteomic analysis of the PE-protein conjugates. Due to its aromatic caging group, 1 is initial-ly concentrating in perinuclear membranes. After uncaging, rapid translocation to mitochondria was observed. Hence, the tool is useful for tracking PE location and for determining the PE interac-tome. A trifunctional phosphatidic acid (PA) derivative in compari-son was rapidly metabolized and is hence more limited in its use.

中文翻译：

---

三功能脂质衍生物：PE 的线粒体相互作用组


磷脂酰乙醇胺 （PE） 是高等真核生物中普遍存在的脂质种类，优先存在于线粒体中。在这里，我们合成了一种多功能 PE 衍生物 （1），用于通过光交联和随后对 PE 蛋白偶联物的分离和蛋白质组学分析来识别完整细胞中的 PE 结合蛋白。由于其芳香族笼基，1 最初集中在核周膜中。解笼后，观察到快速易位到线粒体。因此，该工具可用于跟踪 PE 位置和确定 PE 交互文件。compari-son 中的三功能磷脂酸 （PA） 衍生物被迅速代谢，因此其使用更加有限。