This study presents a novel method for the efficient preparation of peptide-based films through microwave-assisted Lewis acid catalysis (MALC) of buckwheat globulin (BG). The MALC process efficiently degraded BG into small molecular peptides (1.6–1.8 kDa) within 10 min. These peptides formed aggregates with a high β-sheet content through coordination with metal ions, which demonstrated a strong ability to create highly cross-linked network structures. The peptide-based films (PFs), without the addition of any cross-linking agents, exhibited excellent tensile strength (1.65–3.62 MPa), elongation at break (11.52–44.61 %), superior oxygen barrier properties (0.06–2.45 cm³/m²·day), and low swelling rates (20–36 %). Notably, the PFs also possessed strong antibacterial, antioxidant, and ultraviolet resistance capabilities, extending the shelf life of fresh chili peppers by approximately 7 days. This study enhances our understanding of the interactions between transition metal ions and plant proteins, establishing a technological foundation for the large-scale application of plant protein films.

中文翻译：

---

微波辅助路易斯酸催化一步法制备高性能荞麦肽基薄膜的功效、机理和应用


本研究提出了一种通过微波辅助路易斯酸催化 （MALC） 对荞麦球蛋白 （BG） 有效制备肽基膜的新方法。MALC 工艺可在 10 分钟内将 BG 高效降解为小分子肽 （1.6–1.8 kDa）。这些肽通过与金属离子配位形成具有高 β 片含量的聚集体，这显示出创建高度交联网络结构的强大能力。肽基薄膜 （PFs） 在未添加任何交联剂的情况下，表现出优异的拉伸强度 （1.65-3.62 MPa）、断裂伸长率 （11.52-44.61 %）、优异的氧阻隔性能 （0.06-2.45 cm³/m²·day） 和低溶胀率 （20-36 %）。值得注意的是，PFs 还具有很强的抗菌、抗氧化和抗紫外线能力，可将新鲜辣椒的保质期延长约 7 天。这项研究增强了我们对过渡金属离子与植物蛋白之间相互作用的理解，为植物蛋白膜的大规模应用奠定了技术基础。