Benzylisoquinoline alkaloids are significant phyto-nutraceuticals with a bitter flavor. However, due to their complex structures, the sensing and identification of benzylisoquinoline alkaloids is challenging. Benzylisoquinoline alkaloids are commonly extracted from plants such as Coptidis Rhizoma (CR), while the adulteration and dyeing of CR are prevalent. Herein, we innovatively developed a colorimetric sensor array based on the inhibitory effect of alkaloids on urease to identify benzylisoquinoline alkaloids and CR. Urea and pH indicators are used to convert the inhibitory effect of alkaloids on urease into visible signals. Different benzylisoquinoline alkaloids have different inhibition abilities to urease, resulting in their own unique colorimetric response pattern. Combined with linear discriminant analysis (LDA), the colorimetric sensor array has successfully identified different types and concentrations of benzylisoquinoline alkaloids, as well as benzylisoquinoline alkaloid mixtures. The array was further employed to identify CR, encompassing its authenticity, variety, adulteration, and dyeing.

中文翻译：

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一种基于脲酶抑制快速鉴定苄基异喹啉生物碱和黄连根瘤的比色传感器阵列


苄基异喹啉生物碱是重要的植物营养保健品，具有苦味。然而，由于其复杂的结构，苄基异喹啉生物碱的传感和鉴定具有挑战性。苄基异喹啉生物碱通常从黄连 （CR） 等植物中提取，而 CR 的掺假和染色很普遍。在此，我们创新性地开发了一种基于生物碱对脲酶抑制作用的比色传感器阵列，用于鉴定苄基异喹啉生物碱和 CR。尿素和 pH 指示剂用于将生物碱对脲酶的抑制作用转化为可见信号。不同的苄基异喹啉生物碱对脲酶具有不同的抑制能力，从而产生自己独特的比色反应模式。结合线性判别分析 （LDA），比色传感器阵列成功鉴定了不同类型和浓度的苄基异喹啉生物碱，以及苄基异喹啉生物碱混合物。该阵列进一步用于鉴定 CR，包括其真实性、品种、掺假和染色。