The conductivity of Zn-MOF-on-Co-MOF synthesized by one-pot method is improved by searching for the optimum carbonization temperature, which overcomes the limitation of traditional MOF. In order to further enhance electron transfer, the mesoporous PtPdCo trimetal was introduced, which provided considerable load capacity for methylene blue (MB) and reverse complementary DNA (sDNA), and also showed excellent catalytic activity for MB. In this study, the conductivity of aptasensor was improved by modifying carbonized MOF as the base material. In the presence of fipronil (FIP), Apt tended to bind to FIP and was separated from the electrode surface, so that the remaining single-stranded complementary DNA (cDNA) were hybridized with signal probe (MB@PtPdCo/sDNA) and amplified the electrochemical signal. By modifying the substrate, competitive amplification strategy and signal probe material to catalyze signal molecules, the sensor performs multiple signal amplification, and the electrical signal is significantly improved. The sensor has a wide detection range 100 pg/mL to 100 μg/mL and a low detection limit of 76 pg/mL.

中文翻译：

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由集成的 MOF 衍生的 Zn-MOF-on-Co-MOF 偶联的 AuNPs 纳米复合材料和适配体组成的适配体传感器，用于氟虫腈的超灵敏测定


通过寻找最佳碳化温度，通过寻找最佳碳化温度提高了一锅法合成的 Zn-MOF-on-Co-MOF 的电导率，克服了传统 MOF 的局限性。为了进一步增强电子转移，引入了介孔 PtPdCo 三金属，它为亚甲基蓝 （MB） 和反向互补 DNA （sDNA） 提供了相当大的负载能力，并且对 MB 也表现出优异的催化活性。在本研究中，通过改性碳化 MOF 作为基材提高了 aptasensor 的电导率。在氟虫腈 （FIP） 存在下，Apt 倾向于与 FIP 结合并与电极表面分离，因此剩余的单链互补 DNA （cDNA） 与信号探针 （MB@PtPdCo/sDNA） 杂交并放大电化学信号。通过改性衬底、竞争放大策略和信号探针材料催化信号分子，传感器进行多次信号放大，电信号得到显著改善。该传感器具有 100 pg/mL 至 100 μg/mL 的宽检测范围和 76 pg/mL 的低检测限。