Combining nanotechnology with biocatalysts, the construction of a cascade continuous flow reactor is a cutting‐edge strategy to enhance the stability and efficiency of catalysis. In this study, C60@MOF‐545‐Fe nanozyme is synthesized by utilizing fullerene (C60) as a guest encapsulated inside a metal‐organic framework (MOF‐545‐Fe), the unique host‐guest interaction optimizes the oxidase (OXD)‐ and peroxidase (POD)‐like activities, based on which a cascading catalytic strategy is proposed without external energy input. Simultaneously, C60@MOF‐545‐Fe offers the potential to effectively flow at the nanoscale through its unique nanostructure cavity and spatially confined environment. Therefore, the AChE/C60@MOF‐545‐Fe enzyme‐nanozyme continuous flow reactor is constructed by combining C60@MOF‐545‐Fe with acetylcholinesterase (AChE) through supramolecular interactions. Notably, the reactor not only achieves the simultaneous detection of glyphosate, omethoate, and paraoxon but also efficiently differentiates these three organophosphorus pesticides (OPs) by applying the differences in the responses of the three array channels. Subsequently, a portable platform is developed utilizing the YOLO v5‐OPs model based on deep learning, enabling the direct output fitting equation through terminals to achieve rapid point‐of‐care testing (POCT) of OPs. This work not only provides a promising strategy for hazard detection systems but also opens up new avenues for the design of technologies based on flow reactors.

中文翻译：

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酶-纳米酶级联流反应器与深度学习协同作用，用于多种有机磷农药的差异化和即时检测


将纳米技术与生物催化剂相结合，构建复叠式连续流反应器是提高催化稳定性和效率的前沿策略。在这项研究中，利用富勒烯 （C60） 作为封装在金属有机框架 （MOF-545-Fe） 内的客体来合成 C60@MOF-545-Fe 纳米酶，独特的主客体相互作用优化了氧化酶 （OXD） 和过氧化物酶 （POD） 样活性，基于此提出了一种没有外部能量输入的级联催化策略。同时，C60@MOF-545-Fe 提供了通过其独特的纳米结构腔和空间受限环境在纳米尺度上有效流动的潜力。因此，AChE/C60@MOF-545-Fe 酶-纳米酶连续流反应器是通过超分子相互作用将 C60@MOF-545-Fe 与乙酰胆碱酯酶 （AChE） 结合来构建的。值得注意的是，该反应器不仅实现了草甘膦、奥托酸盐和对氧克的同时检测，而且还通过应用三个阵列通道的响应差异有效地区分了这三种有机磷农药 （OP）。随后，利用基于深度学习的 YOLO v5-OPs 模型开发了一个便携式平台，通过终端实现直接输出拟合方程，以实现 OPs 的快速即时检测 （POCT）。这项工作不仅为危害检测系统提供了一种有前途的策略，而且为基于流动反应器的技术设计开辟了新的途径。