Pyocyanin is a pigment that recently gained importance as a valuable chemical. Most often, the method for extraction is based on the use of chloroform. Therefore, an efficient and more environmentally friendly method is vital for further use. We compared organic solvents and ionic liquids as pyocyanin extractants. We assessed the extraction efficiency using a pyocyanin standard and pyocyanin extracted directly from the bacterial culture. The results showed that only chloroform, dichloromethane, and ethyl acetate were useful for pyocyanin extraction among the tested organic solvents. Extraction with chloroform was the most efficient method. Notably, the ionic liquid could also be favorable for the extraction process, exhibiting antibacterial properties and being separated from the pigment by adsorbing pyocyanin on clays and desorbing it with ethanol. The extraction of pyocyanin can be led by using alternative extractants, i.e., dichloromethane or ionic liquid, and therefore, it can be less hazardous for the environment and the user.

中文翻译：

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典型有机溶剂和离子液体的比较，实现更环保、更用户友好的绿脓菌素萃取


绿脓菌素是一种色素，最近作为一种有价值的化学物质而变得重要。大多数情况下，提取方法基于氯仿的使用。因此，一种高效且更环保的方法对于进一步使用至关重要。我们比较了有机溶剂和离子液体作为绿脓菌素萃取剂。我们使用绿脓菌素标准品和直接从细菌培养物中提取的绿脓菌素评估了提取效率。结果表明，在测试的有机溶剂中，只有氯仿、二氯甲烷和乙酸乙酯可用于绿脓菌素的提取。用氯仿萃取是最有效的方法。值得注意的是，离子液体也可能有利于提取过程，表现出抗菌特性，并通过吸附粘土上的绿脓素并用乙醇解吸与颜料分离。绿脓菌素的提取可以通过使用替代萃取剂（即二氯甲烷或离子液体）来引导，因此，它对环境和用户的危害较小。