Osteoarthritis (OA) has long been considered a disease of the articular cartilage. Within the past decade it has become increasingly clear that OA is a disease of the entire joint space and that interactions between articular cartilage and subchondral bone likely play an important role in the disease. Driven by this knowledge, we have created a novel microphysiological model of the osteochondral unit containing synovium, cartilage, bone, and vasculature in separate compartments with molecular and direct cell-cell interaction between the cells from the different tissue types. We have characterized the model in terms of differentiation by molecule and matrix secretion and shown that it demonstrates morphology and functionality that mimic the native characteristic of the joint space. Finally, we induced inflammation and subsequently rescued the model constructs by a known compound as proof of concept for anti-inflammatory drug screening applications.

中文翻译：

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骨软骨单位的血管化微流体模型，用于模拟炎症反应和治疗筛选。


长期以来，骨关节炎 （OA） 一直被认为是关节软骨的一种疾病。在过去的十年中，越来越明显的是 OA 是一种涉及整个关节腔的疾病，关节软骨和软骨下骨之间的相互作用可能在疾病中起着重要作用。在这一知识的推动下，我们创建了一个新的骨软骨单元微生理学模型，其中包含滑膜、软骨、骨骼和脉管系统在单独的隔室中，来自不同组织类型的细胞之间存在分子和直接的细胞间相互作用。我们根据分子和基质分泌的分化来表征该模型，并表明它展示了模拟关节间隙天然特征的形态和功能。最后，我们诱导了炎症，随后通过一种已知化合物挽救了模型构建体，作为抗炎药筛选应用的概念验证。