Excessive reactive oxygen species (ROS) generated by ultraviolet (UV) irradiation significantly contribute to photoaging by increasing the level of matrix metalloproteinases (MMPs), accelerating collagen degradation. Commercial dermal fillers offer temporary wrinkle reduction via volume enhancement. In this study, we propose tilapia-derived collagen hydrogels embedded with ceria nanoparticles (Ce@Col gels) as long-lasting dermal fillers for UVB-induced photoaging. Ceria nanoparticles (CeNPs) significantly enhance the stability of the collagen matrix against enzymatic degradation. These gels exhibit mechanical stability and injectability comparable to those of commercial alternatives. Additionally, CeNPs effectively eliminate ROS to suppress MMP production, curbing both collagen degradation and inflammatory responses. In a UVB-induced photoaging mouse model, the Ce@Col gels significantly reduced the level of oxidative stress in the skin, decreased the number of wrinkles, reduced epidermal thickness, and decreased levels of aging-related biomarkers while increasing the level of collagen deposition. These antiaging effects persisted for seven months post-injection, highlighting Ce@Col gels as a promising approach for prolonged collagen regeneration and sustained anti-inflammatory benefits in photoaged skin.

中文翻译：

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自催化 Ceria 纳米颗粒包埋的罗非鱼胶原蛋白水凝胶作为光老化皮肤的增强抗氧化和持久皮肤填充剂


紫外线 （UV） 照射产生的过量活性氧 （ROS） 通过增加基质金属蛋白酶 （MMP） 的水平，加速胶原蛋白降解，显著促进光老化。市售真皮填充剂通过增容来暂时减少皱纹。在这项研究中，我们提出了包埋氧化铈纳米颗粒 （Ce@Col 凝胶） 的罗非鱼衍生胶原蛋白水凝胶作为 UVB 诱导光老化的长效皮肤填充剂。Ceria 纳米颗粒 （CeNPs） 显着增强胶原蛋白基质对酶降解的稳定性。这些凝胶表现出与商业替代品相当的机械稳定性和可注射性。此外，CeNP 可有效消除 ROS 以抑制 MMP 的产生，抑制胶原蛋白降解和炎症反应。在 UVB 诱导的光老化小鼠模型中，Ce@Col凝胶显著降低了皮肤中的氧化应激水平，减少了皱纹的数量，减少了表皮厚度，降低了与衰老相关的生物标志物水平，同时增加了胶原蛋白沉积的水平。这些抗衰老效果在注射后持续了七个月，突出了Ce@Col凝胶是一种很有前途的方法，可以延长胶原蛋白再生和在光老化皮肤中持续抗炎作用。