Osteoarthritis (OA) is the most common degenerative joint disease, causing pain, disability, and economic strain. Combining nanozymes with dopamine (DOPA)‐based adhesives offers a promising approach to effectively modulate the microenvironment of OA‐affected tissues. However, traditional fabrication methods of DOPA‐based adhesives often involve external oxidants or curing agents, which can introduce additional oxidative stress, posing systemic risks. Here, the different enzyme‐catalyzed activities of platinum–copper (PtCu) nanozymes at different pH values are utilized to directly catalyze the cross‐linking of DOPA‐modified hyaluronic acid (HAD), representing a new polymerization method for phenol‐based bioadhesives. The resulting adhesive (HAD/PtCu/platelet‐rich plasma (PRP)), when combined with PRP, effectively mitigates the generation of proinflammatory factors, scavenges reactive oxygen species, boosts the hypoxic chemotaxis of chondrocytes, stimulates chondrocyte proliferation and migration, and protects interleukin‐1β‐treated human articular chondrocyte C28/I2 cells from further OA advancement. Additionally, in vivo assessments show that HAD/PtCu/PRP significantly alleviates pain and improves the knee microenvironment in osteoarthritic rats. These findings suggest that HAD/PtCu/PRP provides a promising alternative in OA therapy.

中文翻译：

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Nanozyme 工程透明质酸粘合剂加载富含血小板的血浆，用于具有缓解疼痛效果的多层骨关节炎治疗


骨关节炎 （OA） 是最常见的退行性关节疾病，会导致疼痛、残疾和经济压力。将纳米酶与基于多巴胺 （DOPA） 的粘合剂相结合提供了一种有前途的方法，可以有效调节 OA 受影响组织的微环境。然而，基于 DOPA 的胶粘剂的传统制造方法通常涉及外部氧化剂或固化剂，这可能会引入额外的氧化应激，从而带来系统性风险。在这里，利用不同 pH 值下铂-铜 （PtCu） 纳米酶的不同酶催化活性直接催化 DOPA 修饰的透明质酸 （HAD） 的交联，代表了苯酚基生物粘合剂的一种新的聚合方法。所得粘合剂 （HAD/PtCu/富血小板血浆 （PRP）） 与 PRP 结合时，可有效减少促炎因子的产生，清除活性氧，增强软骨细胞的缺氧趋化性，刺激软骨细胞增殖和迁移，并保护白细胞介素-1β 处理的人关节软骨细胞 C28/I2 细胞免受 OA 的进一步发展。此外，体内评估表明，HAD/PtCu/PRP 可显著减轻骨关节炎大鼠的疼痛并改善膝关节微环境。这些发现表明 HAD/PtCu/PRP 为 OA 治疗提供了一种有前途的替代方案。