The bacterial electron transport chain (ETC) plays a crucial role in biofilm energy metabolism and redox balance, making it a potential target for anti‐biofilm treatment. Herein, mesoporous nanocarbon spheres loaded with Pt nanoparticles (CS‐Pt) are developed to disrupt the bacterial ETC for photo‐enhanced anti‐biofilm. Under near‐infrared laser irradiation, CS‐Pt‐treated biofilms show a positive shift of 0.6 eV in Pt 4f binding energy and a 1.76‐fold increase in anodic current density, demonstrating that the electron loss from CS‐Pt results in an enhanced electron transfer from CS‐Pt to biofilm. Adding the electron quencher NH4Cl to the CS‐Pt‐containing biofilm sample results in reduced biofilm clearance, highlighting the critical role of enhanced electron transfer in effective biofilm eradication. Due to the multifunction of CS‐Pt in inducing hyperthermia, reactive oxgen species generation, and interrupting bacterial ETC, the critical component of ETC, extracellular DNA, in methicillin‐resistant Staphylococcus aureus (MRSA) biofilm is reduced by 89.8%, ultimately leading to a biofilm clearance of 90.3%. Additionally, CS‐Pt achieve 99.5% biofilm eradication and expedites wound healing with a rate of 78.1% in a MRSA‐infected wound model. We present an attractive approach for disintegrating biofilm through the synergistic effect of disrupting ETC, enzyme‐like activity, and phototherapy, offering an innovative perspective on combating biofilm‐associated infections.

中文翻译：

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光响应纳米酶破坏细菌电子传递链以增强抗生物膜治疗


细菌电子传递链 （ETC） 在生物膜能量代谢和氧化还原平衡中起着至关重要的作用，使其成为抗生物膜治疗的潜在靶标。在此，开发了载有 Pt 纳米颗粒 （CS-Pt） 的介孔纳米碳球来破坏细菌 ETC 以获得光增强的抗生物膜。在近红外激光照射下，CS-Pt 处理的生物膜显示 Pt 4f 结合能的正偏移 0.6 eV，阳极电流密度增加 1.76 倍，表明 CS-Pt 的电子损失导致从 CS-Pt 到生物膜的电子转移增强。将电子淬灭剂 NH4Cl 添加到含 CS-Pt 的生物膜样品中会导致生物膜清除率降低，突出了增强电子转移在有效根除生物膜中的关键作用。由于 CS-Pt 在诱导体温过高、反应性血原物种生成和中断细菌 ETC 方面的多功能性，耐甲氧西林金黄色葡萄球菌 （MRSA） 生物膜中 ETC 的关键成分，即细胞外 DNA 减少了 89.8%，最终导致生物膜清除率达到 90.3%。此外，在 MRSA 感染的伤口模型中，CS-Pt 可实现 99.5% 的生物膜根除并加速伤口愈合，愈合率为 78.1%。我们提出了一种有吸引力的方法，通过破坏 ETC、酶样活性和光疗的协同作用来分解生物膜，为对抗生物膜相关感染提供了创新视角。