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Overexpression of a high affinity nitrate transporter NtNRT2.1 significantly improves the nicotine content in Nicotiana tabacum
Industrial Crops and Products ( IF 5.6 ) Pub Date : 2024-12-19 , DOI: 10.1016/j.indcrop.2024.120326 Changjian Miao, Zhi Hu, Huijing Ye, Xintong Liu, Yamei Wang, Jinfang Tan, Hongzhen Jiang, Jingguang Chen
Industrial Crops and Products ( IF 5.6 ) Pub Date : 2024-12-19 , DOI: 10.1016/j.indcrop.2024.120326 Changjian Miao, Zhi Hu, Huijing Ye, Xintong Liu, Yamei Wang, Jinfang Tan, Hongzhen Jiang, Jingguang Chen
Nicotine is a crucial alkaloid in tobacco, contributing to 90 % of the total alkaloids. It also acts as a key indicator of the quality of flue-cured tobacco. Typically, nitrate (NO3-) plays a crucial role in the growth and development of plants, and its limited availability has been shown to restrict crop productivity. In this study, we treated tobacco plants with NO3- and ammonium (NH₄⁺), and found that tobacco growth is more dependent on NO3-. In particular, exogenous NO3- application promoted tobacco growth, while also increases nicotine content. Additionally, nicotine content with the nitrate treatment was 5–10 times higher than that of ammonium treatment. Upon further analysis of five tobacco nitrate transporter family (NtNRT2) members, we found that NtNRT2.1 induced by low nitrate, and that the transcription level of NtNRT2.1 is 8–10 times higher than that of other treatments. Likewise, we found that under NO3- treatment, the overexpression (OE) of NtNRT2.1 significantly increases NO3- uptake in the root. However, under low nitrate conditions, the NO3- uptake rate in the OE lines was twice as high as the WT, as well as nicotine content in shoot. Our RT-qPCR analysis also showed that the transcription level of nicotine synthesis and transport relate enzyme genes in WT were approximately 50 % lower than the OE lines with the different nitrate treatments. In summary, our work provides a theoretical basis to develop new high-nicotine tobacco varieties through optimized nutrient utilization.
更新日期：2024-12-20
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