Multiple Strategies Enhance 7-Dehydrocholesterol Production from Kitchen Waste by Engineered Yarrowia lipolytica,Journal of Agricultural and Food Chemistry

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Multiple Strategies Enhance 7-Dehydrocholesterol Production from Kitchen Waste by Engineered Yarrowia lipolytica
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2024-12-19 , DOI: 10.1021/acs.jafc.4c09552
Tianyu Dong, Xiao Zhou, Zheng-Jie Hou, Yujie Shu, Mingdong Yao, Zhi-Hua Liu, Jing-Sheng Cheng, Wenhai Xiao, Ying Wang

7-Dehydrocholesterol (7-DHC) is an important precursor of vitamin D₃. The microbial synthesis of 7-DHC has attracted substantial attention. In this study, multiple strategies were developed to create a sustainable green route for enhancing 7-DHC yield from kitchen waste by engineered Yarrowia lipolytica. Y. lipolytica strains were engineered and combined with various Δ²⁴-dehydrocholesterol reductases. Overexpressing all the genes in the mevalonate pathway improved the precursor pool, increasing the 7-DHC titer from 21.8 to 145.6 mg/L. Additionally, optimizing medium components using the response surface method significantly raised the 7-DHC titer to 391.0 mg/L after shake flask cultivation. The engineered strain yielded a record 7-DHC titer of 3.5 g/L in a 5-L bioreactor when kitchen waste was used as a carbon source. Overall, these results demonstrate that engineered Y. lipolytica efficiently synthesizes 7-DHC from waste lipid feedstock, offering a promising route for its bioproduction.

更新日期：2024-12-20

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