Hypericum perforatum L. (St. John’s Wort) is a medicinal herb used in traditional and modern phytochemical drugs. Usually used to produce phloroglucinol (hyperforin, adhyperforin), naphtodianthrone (hypericin) and xanthone compound extracts for cosmeceuticals and the treatment of mild-moderate depression. The present study shows that H. perforatum inflorescences extracts contain tocopherols (Ts) and rare nature tocotrienols (T3s). To demonstrate that is high probability of the presence of tocotrienols in pharmaceutical products obtained from St. John’s Wort by hydroethanolic solutions, several hydroethanolic extracts (40.0–96.2 %) of H. perforatum inflorescences was obtained. To show that H. perforatum residues can still be a valuable source of tocotrienols after 1st extraction, several extracts using 96.2 % ethanol were produced. From eight tocochromanols present in H. perforatum inflorescences, the extracts contained mostly δ-T3, followed by α-T and α-T3. The 40.0 % and 50.0 % ethanol extracts contained trace amounts of only δ-T3, and the extractability of tocochromanols increased along with the ethanol ratio. Re-extraction in 96.2 % ethanol allowed for complete recovery of δ-T3 from St. John’s Wort. Residues of 1st extraction by hydroethanolic solutions below 70.0 % were a good source of tocotrienols which were successfully re-extracted by using 96.2 % ethanol with the assistance of ultrasound treatment. The present study demonstrated that the pharmaceutical value of H. perforatum is higher than estimated due to the presence of tocotrienols with high bioactive potential, multi-purpose potential of St. John’s Wort biomass, processing chain value improvement (multi-step extraction of bioactive compounds) and reduction of ethanol consumption.

中文翻译：

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贯叶连翘的生育酚和生育三烯酚同系物回收及水乙醇提取后的提取残渣


贯叶连翘 （St. John's Wort） 是一种用于传统和现代植物化学药物的药草。通常用于生产间苯三酚（金丝桃素、adhyperforin）、石脑黄酮（金丝桃素）和氧杂蒽酮复方提取物，用于药妆药和治疗轻中度抑郁症。本研究表明，穿孔胡桃花序提取物含有生育酚 （Ts） 和稀有的生育三烯酚 （T3s）。为了证明通过水乙醇溶液从贯叶连翘中获得的药品中存在生育三烯酚的可能性很高，获得了几种穿孔草花序的水乙醇提取物 （40.0-96.2%）。为了表明穿孔嗜血杆菌残留物在第一次提取后仍然可以成为生育三烯酚的宝贵来源，生产了几种使用 96.2% 乙醇的提取物。在 H. perforatum 花序中存在的 8 个生育色醇中，提取物主要含有 δ-T3，其次是 α-T 和 α-T3。40.0 % 和 50.0 % 乙醇提取物仅含有痕量 δ-T3，生育色胺的可提取性随着乙醇比例的增加而增加。在 96.2 % 乙醇中重新提取可从圣约翰草中完全回收 δ-T3。通过低于 70.0% 的水乙醇溶液进行第一次提取的残留物是生育三烯酚的良好来源，在超声处理的帮助下，使用 96.2% 的乙醇成功重新提取。本研究表明，由于存在具有高生物活性潜力的生育三烯酚，St. 约翰草生物质、加工链价值改进（生物活性化合物的多步提取）和减少乙醇消耗。