Lateolabrax japonicus, a prevalent aquatic delicacy, is known to elicit allergic reactions in certain individuals. Nevertheless, the investigation into its allergenic components has remained notably inadequate. In the research, an approximately 35 kDa heat-stable protein of L. japonicus raw/steamed extracts was verified as tropomyosin (TM) by LC–MS/MS. Open reading frame of TM (852 bp) was acquired via PCR amplification, encoding 284 amino acids. The IgE-binding frequency of TM expressed in Escherichia coli was 22.5% among 80 fish-sensitized patients. Furthermore, TM had the ability to activate basophils in 7 patients. In the Balb/c mice model, compared with the PBS group, the levels of specific antibodies (IgE, IgG1, and IgG2a), CD19⁺ B cells, IL-4, and IL-10 were significantly increased in the TM group. However, the opposite was indeed the case for CD4⁺ TCR-β, CD4⁺ CD25⁺ Fox p 3⁺ cells, and IFN-γ. These findings regarding an allergen assist in conducting component-resolved diagnoses and therapeutic research for fish allergy.

中文翻译：

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原肌球蛋白的鉴定和致敏性分析：Lateolabrax japonicus 中的热稳定过敏原


Lateolabrax japonicus 是一种流行的水生美食，已知会在某些人中引起过敏反应。然而，对其过敏成分的调查仍然明显不足。在研究中，通过 LC-MS/MS 验证了大约 35 kDa 的日本乳杆菌生/蒸提取物的热稳定蛋白为原肌球蛋白 （TM）。通过 PCR 扩增获得 TM 的开放阅读框 （852 bp），编码 284 个氨基酸。在 80 例鱼类致敏患者中，大肠埃希菌中表达的 TM 的 IgE 结合频率为 22.5%。此外，TM 具有激活 7 例患者嗜碱性粒细胞的能力。在 Balb/c 小鼠模型中，与 PBS 组相比，TM 组特异性抗体 （IgE 、 IgG1 和 IgG2a） 、 ^{CD19 +} B 细胞、 IL-4 和 IL-10 水平显著升高。然而，CD4⁺ TCR-β、CD4⁺ CD25⁺ Fox p 3⁺ 细胞和 IFN-γ 的情况确实相反。这些关于过敏原的发现有助于对鱼类过敏进行成分分辨诊断和治疗研究。