Diabetic wounds, often multifactorial and affecting multiple organs, pose substantial challenges to patient well-being, drawing significant interest in biomedical engineering. The demanding wound microenvironment, marked by heightened glucose levels, local exudate, and bacterial infections, emphasizes the pressing demand for advanced wound dressings to meet escalating clinical needs. Herein, a Janus wound dressing with an integration of an antimicrobial hydrophobic nanofiber layer and a 3D hydrophilic sponge was designed and prepared to manage and utilize wound exudate. The hydrophobic layer skillfully combined electrospun poly(ε-caprolactone) (PCL) nanofiber membranes (ENMs) and metal–organic frameworks (MOFs) with peroxidase-like properties by solvent etching, and glucose oxidase (GOx) was grafted through ligand interaction. GOx acts to consume glucose while modulating pH, thus suitable pH and self-supplied H₂O₂ were able to activate the catalytic activity of MOFs to generate ^•OH. Additionally, hydrophilic 3D sponges are constructed using gas foaming technology, which are tactfully combined with hydrophobic ENMs to form a Janus structure, which can transport exudate through the antimicrobial layer to the sponge layer, while sufficient glucose contact with GOx enhances the antimicrobial properties of the designed Janus wound dressing. Experimental results demonstrate the effectiveness of the cascade effect of GOx@PCL/MOF ENMs, ultimately releasing reactive oxygen species and exhibiting robust antibacterial properties. In vivo animal experiments reveal the ability of the Janus wound dressing to mitigate methicillin-resistant Staphylococcus aureus (MRSA) infections in the early stages, thereby expediting the wound healing process. In vivo animal study, the Janus wound dressing achieved a healing rate of 54% on day 3. Our findings underscore the substantial potential of the Janus wound dressings in promoting the healing of chronic diabetic wounds.

中文翻译：

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葡萄糖活化 Janus 伤口敷料用于加强感染和渗出性糖尿病伤口的管理


糖尿病伤口通常是多因素的，影响多个器官，对患者的健康构成重大挑战，引起了人们对生物医学工程的极大兴趣。苛刻的伤口微环境，以葡萄糖水平升高、局部渗出物和细菌感染为特征，强调了对高级伤口敷料的迫切需求，以满足不断升级的临床需求。在此，设计并制备了一种集成了抗菌疏水纳米纤维层和 3D 亲水海绵的 Janus 伤口敷料，以管理和利用伤口渗出物。疏水层通过溶剂刻蚀巧妙地将静电纺丝聚 （ε-己内酯） （PCL） 纳米纤维膜 （ENM） 和具有过氧化物酶样特性的金属有机框架 （MOF） 结合在一起，并通过配体相互作用接枝葡萄糖氧化酶 （GOx）。GOx 在调节 pH 的同时消耗葡萄糖，因此合适的 pH 和自供的 H₂O₂ 能够激活 MOF 的催化活性以生成 ^•OH。此外，亲水性 3D 海绵采用气体发泡技术构建，与疏水性 ENM 巧妙结合，形成 Janus 结构，可以将分泌物通过抗菌层输送到海绵层，而葡萄糖与 GOx 的充分接触增强了设计的 Janus 伤口敷料的抗菌性能。实验结果表明，GOx@PCL/MOF ENMs 的级联效应有效，最终释放活性氧并表现出强大的抗菌特性。 体内动物实验揭示了 Janus 伤口敷料能够在早期减轻耐甲氧西林金黄色葡萄球菌 （MRSA） 感染，从而加速伤口愈合过程。体内动物研究显示，Janus 伤口敷料在第 3 天达到 54% 的愈合率。我们的研究结果强调了 Janus 伤口敷料在促进慢性糖尿病伤口愈合方面的巨大潜力。