Highly sensitive and accurate detection of disease biomarkers is of great importance for diagnosis, staging, and treatment of certain diseases. Herein, we report a novel electrochemical method for the quantification of miRNA biomarkers with DNA tetrahedrons as the signal reporters. Upon the initiation of DNA hairpin opening by miRNA at the electrode interface, the hidden click reaction group is exposed for the bioconjugation with a tetrahedral DNA nanostructure, which carries multiple electrochemical species. Strand displacement polymerization and reductant-mediated amplification are integrated for improved analytical performance. The established method achieves accurate quantitative detection of miRNA in the range from 100 aM to 10 pM. More importantly, it exhibits exceptional selectivity and stability, making it a highly convenient approach to monitor miRNA biomarkers, meeting the requirements of point-of-care testing.

中文翻译：

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基于菌株促进的叠氮化物-炔烃环加成连接的四面体 DNA 纳米标签的 miRNA 电化学定量


疾病生物标志物的高灵敏度和准确检测对于某些疾病的诊断、分期和治疗非常重要。在此，我们报道了一种以 DNA 四面体作为信号报告基因定量 miRNA 生物标志物的新型电化学方法。当 miRNA 在电极界面开始 DNA 发夹开口时，隐藏的点击反应基团被暴露出来，与携带多种电化学种类的四面体 DNA 纳米结构进行生物偶联。将链置换聚合和还原剂介导的扩增相结合，以提高分析性能。所建立的方法实现了对 100 aM 至 10 pM 范围内的 miRNA 的准确定量检测。更重要的是，它表现出卓越的选择性和稳定性，使其成为监测 miRNA 生物标志物的一种非常方便的方法，满足即时检测的要求。