Background

Carbosulfan residues in environment is very harmful to human health. The rapid and high sensitive detection of carbosulfan residues is particularly important to guarantee human health and safety. The conventional chromatographic techniques and enzyme inhibition strategies cannot realize on-site and visual detection of carbosulfan. It is clear that a novel nanozyme-based method was need, which exhibited advantages in low-cost, rapidity, and portability with easy-to-use operation.

Results

A medium entropy-derived flower-like FeCeCu nanozyme (FeCeCuzyme) were prepared via the metal-organic coordination method with “Ostwal ripening” growth process. FeCeCuzyme exhibited an excellent oxidase-like activity due to an abundant active sites in the flower-like structure. Carbosulfan can produce the sulfide compound by hydrolysis reaction under acidic condition, which could easily form sulfide-metal bonds with FeCeCuzyme, thereby significantly quenching their oxidase-like activity and hindering nanozyme-mediated chromogenic reaction. Given this phenomenon, the FeCeCuzyme were used as a colourimetric sensor for highly-specific carbosulfan detection without using acetylcholinesterase and H₂O₂. The FeCeCuzyme colourimetric sensor showed a wide linear range from 0.15 to 50.00 μM ( R² = 0.9970) with low detection limit of 0.13 μM. Meanwhile, FeCeCuzyme sensor was integrated with smartphone to design a portable detection platform, which realizes the on-site and rapid detection of carbosulfan with easy-to-operation. A low detection limit of 0.14 μM was obtained in the linear relation of 0.15 to 50.00 μM. The satisfactory recoveries (91.17%-108.00%) from the spiking method were highly agreed with HPLC technique, and which further verified the feasibility of FeCeCuzyme-based strategies in real samples.

Significance and novelty

This work provides a pioneer new avenue for design of novel nanozymes, and offers an efficient alternative to the conventional methods for specifical, fast, and on-sites detection of carbosulfan in environment.

中文翻译：

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中等熵衍生的花状 FeCeCu 纳米酶，具有优异的氧化酶样活性，用于碳硫丹的现场和目视检测

 背景

环境中的碳百威残留对人体健康非常有害。碳百威残留的快速、高灵敏度检测对于保证人体健康和安全尤为重要。常规的色谱技术和酶抑制策略无法实现对丁硫克百威的现场和目视检测。很明显，需要一种基于纳米酶的新型方法，该方法在低成本、快速、便携和易于使用的操作方面表现出优势。

 结果

通过金属有机配位法和 “Ostwal 成熟 ”生长过程制备了中等熵衍生的花状 FeCeCu 纳米酶 （FeCeCuzyme）。FeCeCuzyme 由于在花样结构中具有丰富的活性位点，因此表现出优异的氧化酶样活性。碳百威在酸性条件下通过水解反应生成硫化物化合物，易与 FeCeCuzyme 形成硫化物-金属键，从而显著淬灭其氧化酶样活性，阻碍纳米酶介导的显色反应。鉴于这种现象，FeCeCuzyme 被用作比色传感器进行高度特异性的碳百丹检测，而无需使用乙酰胆碱酯酶和 H ₂ O ₂ 。FeCeCuzyme 比色传感器显示出 0.15 至 50.00 μM （R ² = 0.9970） 的宽线性范围，检测限低至 0.13 μM。同时，FeCeCuzyme 传感器与智能手机集成，设计了便携式检测平台，实现了对百维安的现场快速检测，操作简单。在 0.15 至 50.00 μM 的线性关系中获得 0.14 μM 的低检测限。加标法的回收率令人满意 （91.17%-108.00%）与 HPLC 技术高度一致，进一步验证了基于 FeCeCuzyme 的策略在实际样品中的可行性。

 意义和新颖性

这项工作为新型纳米酶的设计提供了一条开创性的新途径，并为特异性、快速和现场检测环境中的碳百丹提供了一种有效的替代方案。