Ultra-flexible nanoliposomes (UNL) coated with sodium cholate were fabricated using the thin film hydration technique to encapsulate oleocanthal (OLEO), oleacein (OLEA), oleuropein (OLEU), and hydroxytyrosol (HT) for improving their stability and bioactivity. Their physicochemical properties were further validated through DLS, FTIR, XRD, TGA, and DSC analyses. Negative-staining TEM imaging revealed well-dispersed UNL with laminar vesicles inside. Additionally, their transdermal studies in vitro demonstrated that UNL enhanced the cumulative release of OLEO, OLEA, OLEU, and HT by 3.13, 2.76, 2.59, and 2.83 times, respectively. Furthermore, their release mechanisms were better approximated the Peppas-Sahlin model rather than the Korsmeyer-Peppas and Higuchi models, which governed by Fickian diffusion. Moreover, comparing to their compounds, UNL structure exhibited improved their antioxidant and cytotoxicity properties, highlighting their potential as effective delivery agents in humans. These results offer a novel approach for stabilizing biologically active polyphenols from Olea europaea, paving the way for enhanced human health applications.

中文翻译：

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胆酸盐钠包被的油橄榄多酚纳米脂质体：制备、稳定性、释放和生物活性


使用薄膜水合技术制备了包被胆酸钠的超柔性纳米脂质体 （UNL），以包埋油橄榄素 （OLEO）、油酸甘油酯 （OLEA）、橄榄苦苷 （OLEU） 和羟基酪醇 （HT），以提高它们的稳定性和生物活性。通过 DLS、FTIR、XRD、TGA 和 DSC 分析进一步验证了它们的物理化学性质。负染色 TEM 成像显示分散良好的 UNL，内部有层状囊泡。此外，他们的体外透皮研究表明，UNL 将 OLEO 、 OLEA 、 OLEU 和 HT 的累积释放分别提高了 3.13 倍、 2.76 倍、 2.59 和 2.83 倍。此外，它们的释放机制更接近 Peppas-Sahlin 模型，而不是由 Fickian 扩散支配的 Korsmeyer-Peppas 和 Higuchi 模型。此外，与它们的化合物相比，UNL 结构表现出更好的抗氧化和细胞毒性特性，突出了它们作为人体有效递送剂的潜力。这些结果为稳定油橄榄中的生物活性多酚提供了一种新方法，为增强人类健康应用铺平了道路。