Hen egg-white lysozyme (HEWL) is commonly used in food preservation and as a substitute for sulfur dioxide in wine production and to prevent late blowing defects in cheese production. However, HEWL is an egg allergen that can cause severe allergic reactions in allergic individuals after accidental ingestion. Therefore, in order to prevent life-threatening health problems caused by consumer allergies, we developed an extremely specific gold immunochromatographic assay (GICA) tolerant to matrix effects for the qualification and quantification of HEWL in food products. First, we prepared a monoclonal antibody (mAb) specific for HEWL. By optimizing the parameters of the sandwich enzyme-linked immunosorbent assay, we performed mAb interaction analysis. We developed GICA strips for the determination of HEWL in foods by combining mAb with colloidal gold. Additionally, considering the complex matrix of foods, standard curves for HEWL-negative milk, cheese, and grape wine samples were established to minimize matrix effects. The calculated limits of detection for the determination of HEWL in milk, cheese, and grape wine were 21.12 ng/mL, 0.89 μg/kg, and 1.61 ng/mL, respectively. The recovery rates of the HEWL-spiked samples were 99.16 %—102.25 %, which were comparable to the HPLC results. Thus, we have successfully developed a rapid, sensitive and specific GICA method for the qualification and quantification of HEWL in milk, cheese and grape wine.

中文翻译：

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使用纸质传感器快速、灵敏地定量食品中的鸡蛋清溶菌酶


鸡蛋清溶菌酶 （HEWL） 通常用于食品保鲜，在葡萄酒生产中作为二氧化硫的替代品，并防止奶酪生产中的晚发缺陷。然而，HEWL 是一种鸡蛋过敏原，意外摄入后会导致过敏个体出现严重的过敏反应。因此，为了防止消费者过敏引起的危及生命的健康问题，我们开发了一种对基质效应耐受的极其特异性的金免疫层析法 （GICA），用于食品中 HEWL 的鉴定和定量。首先，我们制备了一种对 HEWL 具有特异性的单克隆抗体 （mAb）。通过优化夹心酶联免疫吸附测定的参数，我们进行了 mAb 相互作用分析。我们开发了 GICA 试纸条，用于将 mAb 与胶体金结合来测定食品中的 HEWL。此外，考虑到食品基质的复杂，建立了 HEWL 阴性牛奶、奶酪和葡萄酒样品的标准曲线，以尽可能减少基质效应。测定牛奶、奶酪和葡萄酒中 HEWL 的计算检测限分别为 21.12 ng/mL、0.89 μg/kg 和 1.61 ng/mL。HEWL 加标样品的回收率为 99.16 %—102.25 %，与 HPLC 结果相当。因此，我们成功开发了一种快速、灵敏和特异的 GICA 方法，用于鉴定和定量牛奶、奶酪和葡萄酒中的 HEWL。