For protein imprinting, template molecule anchoring and imprinting site recognition are very important processes. Herein, we have proposed for the first time to use photo‐switchable host–guest interactions between α‐cyclodextrin (α‐CD)‐modified bovine serum albumin (BSA) and azobenzene coated organic covalent framework (COF) to immobilize and elute proteins. A novel surface labeled polymer microspheres (COF‐MIPs) were constructed, which could reduce the mass transfer resistance and accelerate the adsorption process, resulting in the adsorption equilibrium reaching 60 min and the saturated adsorption amount of 563.86 mg/g. Additionally, using guest molecules (azobenzene) to label the imprinted sites allows the correlation of the relationship between the fluorescence intensity and the protein adsorption process. The fluorescence analyses show that the material exhibits excellent adsorption specificity, with imprinting factors greater than 4. This work extended the application of the host–guest interaction between cyclodextrin and azobenzene and provides a new approach for developing protein‐imprinting materials.

中文翻译：

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通过荧光锚定和主客体相互作用探索 BSA 印迹机制


对于蛋白质印迹，模板分子锚定和印迹位点识别是非常重要的过程。在此，我们首次提出使用 α-环糊精 （α-CD） 修饰的牛血清白蛋白 （BSA） 和偶氮苯包被的有机共价框架 （COF） 之间的光切换主客体相互作用来固定和洗脱蛋白质。构建了一种新的表面标记聚合物微球 （COF-MIPs），可以降低传质阻力并加速吸附过程，使吸附平衡达到 60 min，饱和吸附量为 563.86 mg/g。此外，使用客体分子（偶氮苯）标记印记位点可以实现荧光强度与蛋白质吸附过程之间关系的相关性。荧光分析表明，该材料表现出优异的吸附特异性，印迹因子大于 4。这项工作扩展了环糊精和偶氮苯之间主客体相互作用的应用，并为开发蛋白质印迹材料提供了一种新方法。