Graphene Oxide (GO) nano-sheets have emerged as a potent nanomaterial for a range of applications like antibacterial, antibiofilm. Besides, Microplastic are emerging as a chronic pollutant originated from the aggrandized usage of plastics, for possessing serious repercussions to the living beings and the environment. In concern of the issue, the individual toxicological impacts of GO nano-sheets and Polystyrene (PS) have received substantial research, the mechanistic details and toxicological effects of GO and PS as a hybrid is yet unknown. This study evaluates the in vivo biotoxicity of a lab mimic green synthesized GO@PS hybrid using embryonic zebrafish through experimental and computational approach. The physiochemical characterzation of the GO@PS verified the synthesis of a stable 1433.0 ± 268.0 nm sized GO@PS hybrid with a zeta potential of -47.3 ± 5.7 mV. Mechanistic analysis deduced the toxicological impact as a cause of induced apoptosis due to dysregulated oxidative stress lead by the hypoxic condition created due to blockage of chorion by attachment and accumulation of GO@PS. The study depicted the in vivo toxicity of GO, PS and GO@PS at cellular and molecular level to draw attention for taking measures in usage of GO and PS in terms of environmental and human health.

中文翻译：

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揭示由近端本征原子相互作用介导的绿色生物制造氧化石墨烯-微塑料杂化物的体内生物毒性


氧化石墨烯 （GO） 纳米片已成为一种有效的纳米材料，可用于抗菌、抗生物膜等一系列应用。此外，微塑料正在成为一种慢性污染物，源于塑料的大量使用，对生物和环境造成严重影响。就此问题而言，GO 纳米片和聚苯乙烯 （PS） 的个体毒理学影响已接受大量研究，GO 和 PS 作为杂交物的机理细节和毒理学影响尚不清楚。本研究通过实验和计算方法评估了使用胚胎斑马鱼合成的实验室模拟绿色合成GO@PS杂交种的体内生物毒性。GO@PS的理化特性验证了稳定的 1433.0 ± 268.0 nm 大小的 GO@PS 杂化物的合成，zeta 电位为 -47.3 ± 5.7 mV。机制分析推断毒理学影响是诱导细胞凋亡的原因，这是由于氧化应激失调引起的，这是由于GO@PS的附着和积累阻塞绒毛膜而产生的缺氧条件。该研究描述了 GO、PS 和 GO@PS 在细胞和分子水平上的体内毒性，以引起人们对在环境和人类健康方面采取措施使用 GO 和 PS 的关注。